高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物

引用本文: 邹游, 邵琳智, 吴映璇. 高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物[J]. 色谱, 2019, 37(10): 1112-1117. doi: 10.3724/SP.J.1123.2019.03031 shu

Citation: ZOU You, SHAO Linzhi, WU Yingxuan. Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(10): 1112-1117. doi: 10.3724/SP.J.1123.2019.03031 shu

高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物

广东检验检疫技术中心, 广东广州 510623
基金项目:
农业行业标准制定和修订项目（2017-108）.

摘要: 建立了高效液相色谱同时测定鸡肉、鸡肝脏和鸡肾脏中11种苯并咪唑类药物残留标志物的方法。样品采用乙腈提取，经正己烷脱脂后以MCX固相萃取小柱净化，净化液浓缩处理后采用Atlantis T₃色谱柱分离，以甲醇-1%（v/v）乙酸水溶液为流动相进行梯度洗脱，紫外检测波长为292 nm，外标法定量检测。目标化合物在25~1000 μg/L范围内线性良好，定量限为50 μg/kg；在不同添加水平下目标化合物的回收率为70.91%~103.21%，相对标准偏差为1.48%~10.08%（n=6），精密度和准确度均符合要求，能够满足鸡组织中苯并咪唑类药物残留标志物的检测要求。

关键词:

English

Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography

Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China
Received Date: 25 March 2019
Fund Project:
Project of Agricultural Industry Standards Development and Revision (No. 2017-108).

Abstract: A method was developed for the simultaneous determination of 11 benzimildazoles residue markers in chicken meat, liver and kidney samples by high performance liquid chromatography (HPLC). The chicken tissue sample was extracted with acetonitrile, defatted with n-hexane, and purified with an MCX solid-phase extraction cartridge. Chromatographic separation was performed on an Atlantis T₃ column with the gradient elution of methyl alcohol-1% (v/v) acetic acid aqueous solution as the mobile phases at a flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm. Analytes were quantified by the external standard method. The linearities of the calibration curves were well in the range of 25-1000 μg/L, and the limit of quantification was 50 μg/kg. The recoveries at different spiked levels in all the three matrices ranged from 70.91% to 103.21%, and the relative standard deviations (RSDs) were between 1.48% and 10.08% (n=6). The developed method is characterized by an excellent purification effect, sensitivity and accuracy, and can be used for the determination of benzimidazoles residue markers in chicken tissue samples.

Key words:

high performance liquid chromatography (HPLC)
/ solid phase extraction(SPE)
/ benzimidazoles residue markers
/ chicken tissue

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高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物

English

Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography

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中国化学会秘书处

高效液相色谱法测定鸡组织中苯并咪唑类药物残留标志物

English

Determination of benzimidazoles residue markers in chicken tissue by high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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