分散固相萃取结合气相色谱-高分辨质谱快速筛查酱油中多种氯丙醇

引用本文: 黄超群, 陈钦可, 陈丽, 王云宇, 马喆, 沈咪, 黄佳波. 分散固相萃取结合气相色谱-高分辨质谱快速筛查酱油中多种氯丙醇[J]. 色谱, 2019, 37(10): 1048-1052. doi: 10.3724/SP.J.1123.2019.03016 shu

Citation: HUANG Chaoqun, CHEN Qinke, CHEN Li, WANG Yunyu, MA Zhe, SHEN Mi, HUANG Jiabo. Determination of multiple chloropropanols in soy sauce by dispersive solid phase extraction-gas chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(10): 1048-1052. doi: 10.3724/SP.J.1123.2019.03016 shu

分散固相萃取结合气相色谱-高分辨质谱快速筛查酱油中多种氯丙醇

浙江省检验检疫科学技术研究院, 浙江杭州 310016

摘要: 建立了酱油中1，3-二氯-2-丙醇、2，3-二氯-1-丙醇、3-氯-1，2-丙二醇和2-氯-1，3-丙二醇的分散固相萃取-气相色谱-高分辨质谱快速筛查检测方法。样品采用乙酸乙酯提取，提取液浓缩后经N-丙基乙二胺净化，气相色谱-高分辨质谱测定，内标法定量。结果表明，该方法对于酱油中4种氯丙醇的定量限为0.5~10 μg/kg。在3个浓度水平下的加标回收率为78%~103%；相对标准偏差均不大于8.8%。该方法快速、简便、准确、灵敏，可作为酱油中4种氯丙醇的有效检测方法。

关键词:

English

Determination of multiple chloropropanols in soy sauce by dispersive solid phase extraction-gas chromatography-high resolution mass spectrometry

Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China
Received Date: 14 March 2019

Abstract: A method for the rapid screening of 1,3-dichloro-2-propanol, 2,3-dichloro-1-propanol,3-monochloropropane-1,2-diol, and 2-monochloropropane-1,3-diol in soy sauce was developed based on dispersive solid phase extraction-gas chromatography-high resolution mass spectrometry (GC-HRMS). The samples were extracted by ethyl acetate, cleaned up using N-propylethylenediamine and detected by GC-HRMS. The chloropropanols were quantified by the internal standard method. The results demonstrated that the limits of quantitation of the four chloropropanols were in the range of 0.5-10 μg/kg. The recoveries of the four chloropropanols in soy sauce spiked with three levels varied from 78% to 103%, and the relative standard deviations were no more than 8.8%. The proposed method is simple, accurate, sensitive and suitable for the rapid screening of the four chloropropanols in soy sauce.

Key words:

dispersive solid phase extraction
/ gas chromatography-high resolution mass spectrometry (GC-HRMS)
/ chloropropanol
/ soy sauce

1. [1] Crews C. Encyclop Food Safety, 2014, 2:392
2. [2] Sameer A, Maciej J B, Zuhoor I, et al. Food Cont, 2007, 18(1):81
3. [3] GB 2762-2017
4. [4] CXS 193-1995
5. [5] European Commission. Commission Regulation No 1881/2006. (2006-12-19)[2019-08-13]. https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32006R1881&qid=1565663728766&from=EN
6. [6] GB 5009.191-2016
7. [7] Racamonde I, Gonzalez P, Lorenzo R A, et al. J Chromatogr A, 2011, 1218:6878
8. [8] Carro A M, Gonzalez P, Lorenzo R A. J Chromatogr A, 2013, 1319:35
9. [9] Qiu X Z, Xu X Y, Liang Y, et al. Food Chem, 2018, 258:295
10. [10] Lin X S, Wu H Q, Hang Y P, et al. Journal of Instrumental Analysis, 2012, 31(2):143 林晓珊, 吴慧勤, 杭义萍, 等. 分析测试学报, 2012, 31(2):143
11. [11] Mo W M, He H L, Xu X M, et al. Food Cont, 2014, 43:251
12. [12] Yi Q, Miao H, Wu Y N. Chinese Journal of Analytical Chemistry, 2016, 44(5):678 易青, 苗虹, 吴永宁. 分析化学, 2016, 44(5):678
13. [13] Pan X B, Yi X H, Shi Y J, et al. Chinese Journal of Analytical Chemistry, 2016, 44(7):1125 潘孝博, 伊雄海, 时逸今, 等. 分析化学, 2016, 44(7):1125
14. [14] Li R, He C M, Yang L Q, et al. Chinese Journal of Chromatography, 2017, 35(8):808 李蓉, 何春梅, 杨璐齐, 等. 色谱, 2017, 35(8):808
15. [15] Zhang X C, Li R, Zhang P J, et al. Chinese Journal of Chromatography, 2018, 36(2):114 张宪臣, 李蓉, 张朋杰, 等. 色谱, 2018, 36(2):114
16. [16] Zhang R, Liu X, Peng Y, et al. Chinese Journal of Chromatography, 2018, 36(12):1228 张蓉, 刘鑫, 彭媛, 等. 色谱, 2018, 36(12):1228
17. [17] Michelangelo A, Steven J L. J AOAC Int, 2003, 86(2):412
18. [18] Jiang Y, Ni Y N, Zhu H F. Journal of Nanchang University, 2006, 30(1):40 江勇, 倪永年, 朱惠芳. 南昌大学学报, 2006, 30(1):40

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分散固相萃取结合气相色谱-高分辨质谱快速筛查酱油中多种氯丙醇

English

Determination of multiple chloropropanols in soy sauce by dispersive solid phase extraction-gas chromatography-high resolution mass spectrometry

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中国化学会秘书处

分散固相萃取结合气相色谱-高分辨质谱快速筛查酱油中多种氯丙醇

English

Determination of multiple chloropropanols in soy sauce by dispersive solid phase extraction-gas chromatography-high resolution mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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