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外泌体分离技术及其临床应用研究进展

高方园 ,  焦丰龙 ,  张养军 ,  秦伟捷 ,  钱小红

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引用本文: 高方园,  焦丰龙,  张养军,  秦伟捷,  钱小红. 外泌体分离技术及其临床应用研究进展[J]. 色谱, 2019, 37(10): 1071-1083. doi: 10.3724/SP.J.1123.2019.02007 shu
Citation:  GAO Fangyuan,  JIAO Fenglong,  ZHANG Yangjun,  QIN Weijie,  QIAN Xiaohong. Advances in separation techniques for exosomes and their clinical applications[J]. Chinese Journal of Chromatography, 2019, 37(10): 1071-1083. doi: 10.3724/SP.J.1123.2019.02007 shu

外泌体分离技术及其临床应用研究进展

  • 军事科学院军事医学研究院生命组学研究所, 蛋白质组学国家重点实验室, 北京蛋白质组研究中心, 国家蛋白质科学中心-北京, 北京 102206

  • 基金项目:

    国家重点研发计划（2017YFA0505002，2018YFC0910302，2016YFA0501403，2018YFF0212505，2017YFC0906703）.

摘要: 外泌体是细胞通过胞吐过程分泌的一类粒径为30~200 nm的囊泡，其组成包括脂质双分子层以及其内部包裹的细胞来源的蛋白质、核糖核苷酸（RNA）和脱氧核糖核苷酸（DNA）等生物分子。作为一种细胞间交流的重要方式，外泌体在一系列生理和病理过程中起着至关重要的作用。由于体液环境复杂，加之自身体积小、密度低，外泌体的富集与分离对于其后续分析和功能研究至关重要。该文介绍了外泌体的研究策略、表征手段及生物学功能和临床应用研究进展，特别对外泌体的提取方法进行了详细介绍，并加以系统评述。

English

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  • 收稿日期:  2019-02-20
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