基于液相色谱-高分辨质谱的代谢组学技术用于麦卢卡蜂蜜的甄别

引用本文: 严丽娟, 徐敦明, 薛晓锋, 林立毅, 赖康, 王建文, 张志刚. 基于液相色谱-高分辨质谱的代谢组学技术用于麦卢卡蜂蜜的甄别[J]. 色谱, 2019, 37(6): 589-596. doi: 10.3724/SP.J.1123.2018.12011 shu

Citation: YAN Lijuan, XU Dunming, XUE Xiaofeng, LIN Liyi, LAI Kang, WANG Jianwen, ZHANG Zhigang. Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique[J]. Chinese Journal of Chromatography, 2019, 37(6): 589-596. doi: 10.3724/SP.J.1123.2018.12011 shu

基于液相色谱-高分辨质谱的代谢组学技术用于麦卢卡蜂蜜的甄别

严丽娟 ^1, ,
徐敦明 ^1, ,
薛晓锋 ^2, ,
林立毅 ^1, ,
赖康 ^3, ,
王建文 ^3, ,
张志刚 ^1,

1.
厦门海关检验检疫技术中心, 福建厦门 361026;
2.
中国农业科学院蜜蜂研究所, 北京 100093;
3.
四川省蜂业管理站, 四川成都 610042
基金项目:
国家重点研发计划项目（2017YFC1601600）；福建省自然科学基金（2016J01071）；厦门市科技惠民项目（3502Z20174043）；福建省社会发展引导性（重点）项目（2017Y0077）；原质检总局科技项目（2016IK184）.

摘要: 采用基于液相色谱-高分辨质谱的代谢组学技术对麦卢卡蜂蜜以及国内主要蜂蜜品种的代谢谱进行全面分析，实现了麦卢卡蜂蜜与其他蜂蜜的区分，建立了偏最小二乘法判别模型，对测试样品能够取得很好的鉴定效果。实验从麦卢卡蜂蜜中筛选出高度表达的3-苯乳酸、甲氧基苯乙酮、二氢麻醉椒苦素、芹菜素等黄酮和肉桂酸类等34种代谢标志物，所构建的标志物组合模型的受试者工作特征曲线下的面积达到了0.99。该文建立的代谢组学方法为麦卢卡蜂蜜的质量控制提供了新的思路。

关键词:

English

Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique

1.
Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, China;
2.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
3.
Sichuan Province Apiculture Management Station, Chengdu 610042, China
Received Date: 06 December 2018
Fund Project:
National Key Research and Development Program of China (No. 2017YFC1601600)

Abstract: A liquid chromatography-high resolution mass spectrometry (LC-HRMS) based metabolomic approach for the discrimination of manuka honey from the domestic honey was developed. The results of multivariate data analysis showed significant discrimination of manuka honey from domestic honey. Furthermore, a partial least squares discrimination model was established for honey discrimination, enabling the correct identification of unknown testing samples. A total of 34 highly expressed metabolic markers were obtained from the developed discrimination model, including 3-phenyllactic acid and 4-hydroxybenzoic acid which had both been previously identified as manuka honey markers. The area under the curve of the constructed marker combination reached 0.99. The developed metabolomic approach provides a new method for the quality control of manuka honey.

Key words:

liquid chromatography (LC)
/ high resolution mass spectrometry (HRMS)
/ metabolomics
/ manuka honey

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基于液相色谱-高分辨质谱的代谢组学技术用于麦卢卡蜂蜜的甄别

English

Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique

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中国化学会秘书处

基于液相色谱-高分辨质谱的代谢组学技术用于麦卢卡蜂蜜的甄别

English

Authenticity identification of manuka honey using liquid chromatography-high resolution mass spectrometry based metabolomic technique

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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