利用“网包法”制备高效液相色谱大环抗生素类手性固定相

引用本文: 何义娟, 李克丽, 李倩, 张鹏, 艾萍, 袁黎明. 利用“网包法”制备高效液相色谱大环抗生素类手性固定相[J]. 色谱, 2019, 37(4): 383-391. doi: 10.3724/SP.J.1123.2018.08035 shu

Citation: HE Yijuan, LI Keli, LI Qian, ZHANG Peng, AI Ping, YUAN Liming. Preparation of macrocycle antibiotic chiral stationary phases by wrapping with a net in high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(4): 383-391. doi: 10.3724/SP.J.1123.2018.08035 shu

利用“网包法”制备高效液相色谱大环抗生素类手性固定相

云南师范大学化学化工学院, 昆明 650500
基金项目:
国家自然科学基金项目（21365025）.

摘要: 万古霉素和替考拉宁都属于糖肽类的大环抗生素，具有立体的环状结构和多个手性中心，是两种常见的手性识别材料，广泛应用于对映体的色谱手性分离分析。该文以万古霉素和替考拉宁为手性选择剂，哌嗪为单体，4，4'-二苯基甲烷二异氰酸酯（MDI）、1，6-己二异氰酸酯（HDI）和2，4-甲苯二异氰酸酯（TDI）为交联剂，通过界面聚合反应形成网状层包裹硅胶载体的方法制得6种高效液相色谱手性固定相，用于分离外消旋化合物，并与MDI直接交联万古霉素和替考拉宁在硅胶表面所得固定相进行了比较。结果表明，利用"网包法"和直接交联法制备的手性柱与商品万古霉素和替考拉宁柱之间具有互补性，均对不同的外消旋体有不同程度的拆分。

关键词:

English

Preparation of macrocycle antibiotic chiral stationary phases by wrapping with a net in high performance liquid chromatography

School of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China
Received Date: 23 August 2018
Fund Project:
National Natural Science Foundation of China (No. 21365025).

Abstract: Vancomycin and teicoplanin are glycopeptide macrocyclic antibiotics with three-dimensional ring structures and multiple chiral centers. They are commonly used chiral recognition materials in enantioseparation by high-performance liquid chromatography. In this study, six chiral stationary phases wrapped with a net on silica gels were prepared by interfacial polymerization using vancomycin and teicoplanin as chiral selectors, piperazine as a monomer, 4,4'-diphenylmethane diisocyanate (MDI), 1,6-hexamethylene diisocyanate (HDI), and 2,4-toluene diisocyanate (TDI) as crosslinker. The fabricated chiral columns were used for enantioseparations. Compared to the crosslinked and commercial columns composed of vancomycin and teicoplanin, the columns wrapped with a net exhibited complementarity for the optical resolutions.

Key words:

high performance liquid chromatography (HPLC)
/ chiral stationary phase
/ interfacial polymerization
/ macrocycle antibiotic

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沈阳化工大学材料科学与工程学院沈阳 110142

利用“网包法”制备高效液相色谱大环抗生素类手性固定相

English

Preparation of macrocycle antibiotic chiral stationary phases by wrapping with a net in high performance liquid chromatography

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中国化学会秘书处

利用“网包法”制备高效液相色谱大环抗生素类手性固定相

English

Preparation of macrocycle antibiotic chiral stationary phases by wrapping with a net in high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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