复合免疫亲和柱-高效液相色谱法同时测定谷物及其制品中9种真菌毒素

引用本文: 王韦岗, 强敏, 端礼钦. 复合免疫亲和柱-高效液相色谱法同时测定谷物及其制品中9种真菌毒素[J]. 色谱, 2018, 36(12): 1330-1336. doi: 10.3724/SP.J.1123.2018.08008 shu

Citation: WANG Weigang, QIANG Min, DUAN Liqin. Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column[J]. Chinese Journal of Chromatography, 2018, 36(12): 1330-1336. doi: 10.3724/SP.J.1123.2018.08008 shu

复合免疫亲和柱-高效液相色谱法同时测定谷物及其制品中9种真菌毒素

1.
镇江市产品质量监督检验中心, 江苏镇江 212132;
2.
徐州市农水畜禽产品质量检测中心, 江苏徐州 221006

摘要: 建立了复合免疫亲和柱-在线光化学衍生-高效液相色谱同时测定谷物及其制品中9种真菌毒素的检测方法。以乙腈-水（80：20，v/v）混合溶液提取样品中9种真菌毒素，提取液经自制真菌毒素复合免疫亲和柱净化，采用高效液相色谱进行分离，在线光化学衍生后进入荧光检测器测定，外标法定量。结果表明，9种真菌毒素在相应浓度范围内线性关系良好，相关系数均大于0.999；在低、中、高3个不同加标浓度下，9种真菌毒素的回收率均大于80%，相对标准偏差（RSD）为1.0%~5.6%；方法的检出限（LOD）为0.02~5.00 μg/kg，定量限（LOQ）为0.07~16.70 μg/kg。该方法具有重现性好、灵敏度高、结果准确的特点，适用于谷物及其制品中9种真菌毒素残留的分析检测。

关键词:

English

Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column

1.
Zhenjiang Center for Products Quality Supervision and Inspection, Zhenjiang 212132, China;
2.
Xuzhou Quality Inspection and Testing Center for Agricultural Products, Xuzhou 221006, China
Received Date: 06 August 2018

Abstract: A rapid analytical method using high performance liquid chromatography (HPLC) with post-column derivatization was established for the simultaneous determination of nine mycotoxins in cereal and cereal products. The nine mycotoxins were extracted from the samples by using acetone-water (80:20, v/v). The extract was purified by a self-made composite immunoaffinity clean-up column and was analyzed by HPLC. Mycotoxins were detected by fluorescence detection after on-line photochemical derivatization and were quantified by an external standard method. The nine compounds showed good linearities in their concentration ranges with the correlation coefficients (R²) exceeding 0.999. The spiked recoveries in real samples for the nine mycotoxins were above 80%, while the relative standard deviations (RSDs) were in the range of 1.0%-5.6%. The limits of detection (LODs) and the limits of quantification (LOQs) were 0.02-5.00 and 0.07-16.70 μg/kg, respectively. The developed method is simple, sensitive, and has good repeatability, and thus can be used for the routine analysis of the nine mycotoxins in cereal and cereal products.

Key words:

high performance liquid chromatography (HPLC)
/ composite immunoaffinity column
/ mycotoxin
/ cereal and cereal products

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

复合免疫亲和柱-高效液相色谱法同时测定谷物及其制品中9种真菌毒素

English

Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

复合免疫亲和柱-高效液相色谱法同时测定谷物及其制品中9种真菌毒素

English

Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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