改进的高效液相色谱法测定婴幼儿配方奶粉中5种核苷酸

引用本文: 励炯, 王姣斐, 邱红钰, 李玮. 改进的高效液相色谱法测定婴幼儿配方奶粉中5种核苷酸[J]. 色谱, 2017, 35(8): 832-836. doi: 10.3724/SP.J.1123.2017.04007 shu

Citation: LI Jiong, WANG Jiaofei, QIU Hongyu, LI Wei. Determination of five nucleotides in infant formula milk powder by modified high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2017, 35(8): 832-836. doi: 10.3724/SP.J.1123.2017.04007 shu

改进的高效液相色谱法测定婴幼儿配方奶粉中5种核苷酸

杭州市食品药品检验研究院, 浙江杭州 310017

摘要: 建立并优化了高效液相色谱检测婴幼儿配方奶粉中5种核苷酸（尿嘧啶核苷酸（UMP）、腺嘌呤核苷酸（AMP）、次黄嘌呤核苷酸（IMP）、鸟嘌呤核苷酸（GMP）、胞嘧啶核苷酸（CMP））的方法。样品用水提取后，经乙酸沉淀蛋白质和HLB固相萃取柱净化，采用Waters XBrigde Amide（150 mm×4.6 mm，3.5 μ m）色谱柱分离，以乙腈、10 mmol/L磷酸二氢钠溶液和0.12%（v/v）磷酸溶液为流动相进行梯度洗脱，二极管阵列检测器（波长为254 nm）检测。结果表明，5种核苷酸检测的线性范围宽，相关性好，相关系数（r²）均为0.9999；方法的加标回收率为86.9%~105.7%；定量限为5.6~8.0 mg/kg；日内和日间精密度分别为0.5%~1.7%（n=5）和0.6%~1.9%（n=9）。该法前处理简单，分离效果好，回收率高，重复性好，可作为婴幼儿配方奶粉中5种核苷酸的有效检测方法。

关键词:

English

Determination of five nucleotides in infant formula milk powder by modified high performance liquid chromatography

Hangzhou Institute for Food and Drug Control, Hangzhou 310017, China
Received Date: 06 April 2017

Abstract: A modified high performance liquid chromatographic (HPLC) method was developed for the determination of the five nucleotides (uridine monophosphate (UMP), adenosine monophosphate (AMP), inosine monophosphate (IMP), guanosine monophosphate (GMP) and cytidine monophosphate (CMP)) in infant formula milk powder. The samples were extracted by water, deproteinized by acetic acid and purified with an HLB SPE cartridge. The analytes were separated by a Waters XBrigde Amide column (150 mm×4.6 mm, 3.5 μ m). Acetonitrile, 10 mmol/L sodium dihydrogen phosphate aqueous solution and 0.12%(v/v) phosphoric acid aqueous solution were used as mobile phases with gradient elution. The detection wavelength of photodiode array detector was set at 254 nm. Five linear calibration curves were obtained with correlation coefficients (r²) of 0.9999. The recoveries were determined at three spiked levels ranging from 86.9% to 105.7%. The limits of quantification (LOQs) were from 5.6 mg/kg to 8.0 mg/kg. The intra-day and inter-day precisions were 0.5%-1.7% (n=5) and 0.6%-1.9% (n=9), respectively. The method is simple, effective, accurate and repeatable. It is suitable for thedetermination of the five nucleotides in infant formula milk powder.

Key words:

high performance liquid chromatography (HPLC)
/ solid phase extraction (SPE)
/ nucleotide
/ infant formula milk powder

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沈阳化工大学材料科学与工程学院沈阳 110142

改进的高效液相色谱法测定婴幼儿配方奶粉中5种核苷酸

English

Determination of five nucleotides in infant formula milk powder by modified high performance liquid chromatography

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改进的高效液相色谱法测定婴幼儿配方奶粉中5种核苷酸

English

Determination of five nucleotides in infant formula milk powder by modified high performance liquid chromatography

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