LTQ-Orbitrap组合式高分辨质谱法快速筛查毛发中7种毒品及代谢物

引用本文: 叶海英, 郑水庆, 梁晨, 汪蓉, 张玉荣, 张凌怡, 张维冰, 张润生. LTQ-Orbitrap组合式高分辨质谱法快速筛查毛发中7种毒品及代谢物[J]. 分析化学, 2012, 40(11): 1674-1679. doi: 10.3724/SP.J.1096.2012.20244 shu

Citation: YE Hai-Ying, ZHENG Shui-Qing, LIANG Chen, WANG Rong, Zhang Yu-Rong, ZHANG Ling-Yi, Zhang Wei-Bing, ZHANG Run-Sheng. Analysis of Seven Drugs in Human Hair by Hybrid Linear Ion Trap/Orbitrap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1674-1679. doi: 10.3724/SP.J.1096.2012.20244 shu

LTQ-Orbitrap组合式高分辨质谱法快速筛查毛发中7种毒品及代谢物

叶海英 ^1, ,
郑水庆 ^1, ,
梁晨 ^1, ,
汪蓉 ^1, ,
张玉荣 ^1, ,
张凌怡 ^2, ,
张维冰 ^2, ,
张润生 ^{1,
,}

通讯作者: 张润生

基金项目:
本文系公安部应用创新计划(No. 2009YYCXSHSJ033)资助项目 (No. 2009YYCXSHSJ033)

摘要: 采用超高压液相色谱-二维线性离子阱结合静电场轨道阱组合式高分辨质谱联用技术(Accela U-HPLC/LTQ Orbitrap XL), 建立了人毛发中吗啡、O⁶-单乙酰吗啡、可待因、乙酰可待因、氯胺酮、去甲氯胺酮和美沙酮毒品及代谢物快速筛查方法。取毛发样品经表层清洗后冷冻研磨粉碎, 置于硼酸盐缓冲液(pH 9.2)中超声90 min, 离心取上清液, 用Oasis HLB柱固相萃取制备。通过静电场轨道阱全扫描得到毒品及其代谢物的精确相对分子质量, 同时进行7种毒品及其代谢物的快速筛查。高分辨率质谱可有效去除毛发基质干扰, 毒品及其代谢物筛查检出限在0.001-0.02 ng/mg, 在0.05-50 ng/mg范围内存在良好线性关系(r>0.9975);本方法平均加标回收率为76.1%-109.6%;日内及日间精密度RSD≤14.9%。本方法灵敏度高, 样品制备简便, 适用于常见毒品的快速筛查。

关键词:

English

Analysis of Seven Drugs in Human Hair by Hybrid Linear Ion Trap/Orbitrap Mass Spectrometry

1.
¹ Shanghai Institiute of Forensic Science, Shanghai Key Laboratory of Crime Scene Evidence-State Key Laboratory Breeding Base of Crime Scene Evidence, Shanghai, 200083 China;

Corresponding author: 张润生

Received Date: 12 March 2012
Fund Project:
本文系公安部应用创新计划(No. 2009YYCXSHSJ033)资助项目

Abstract: A new method was developed for the determination of seven illicit drugs and metabolites in human hair by ultra high performance liquid chromatograph tandem a linear ion trap-orbitrap hybrid instrument at high mass resolution (UHPLC-LTQ-Orbitrap/MS). After being washed and grinded, the hair samples were soaked in pH 9.2 borate buffer with ultrasonic for 90 min. Then the samples were extracted by solid phase extraction (SPE). High resolution mass spectrometry was applied to reduce matrix interference. Full scanning of electrostatic field orbitrap was applied for quantification of drugs. The limits of detection (LOD) of seven illicit drugs and metabolites in human hair were 0.001-0.02 μg/g. The method showed a fairly good linearity over the range of 0.05-50 μg/g (r>0.9975). The spiked recoveries were between 76.1%-109.6%, and the deviation of intra-and inter-day precision was less than 14.9%. The method is simple, high sensitive, and suitable for the determination of illicit drugs in human hair.

Key words:

Linear ion trap/orbitrap mass spectrometry
/ Opiates
/ Ketamine
/ Methadone
/ Solid phase extraction
/ Ultra high performance liquid chromatography
/ Hair

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

LTQ-Orbitrap组合式高分辨质谱法快速筛查毛发中7种毒品及代谢物

通讯作者: 张润生

English

Analysis of Seven Drugs in Human Hair by Hybrid Linear Ion Trap/Orbitrap Mass Spectrometry

Corresponding author: 张润生

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

LTQ-Orbitrap组合式高分辨质谱法快速筛查毛发中7种毒品及代谢物

通讯作者: 张润生

English

Analysis of Seven Drugs in Human Hair by Hybrid Linear Ion Trap/Orbitrap Mass Spectrometry

Corresponding author: 张润生

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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