伏马菌素B1单克隆抗体制备及化学发光免疫检测方法的建立

许杨 邹龙 刘师文 刘京 陈波

引用本文: 许杨, 邹龙, 刘师文, 刘京, 陈波. 伏马菌素B1单克隆抗体制备及化学发光免疫检测方法的建立[J]. 分析化学, 2012, 40(11): 1735-1739. doi: 10.3724/SP.J.1096.2012.20201 shu
Citation:  XU Yang, ZOU Long, LIU Shi-Wen, LIU Jing, CHEN Bo. Production of Monoclonal Antibodies for Detection of Fumonisin B1 by Chemiluminescence Enzyme-linked Immunosorbent Assaay[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1735-1739. doi: 10.3724/SP.J.1096.2012.20201 shu

伏马菌素B1单克隆抗体制备及化学发光免疫检测方法的建立

    通讯作者: 许杨
  • 基金项目:

    本文系国家"863"计划资助项目(No. 2007AA10Z427) (No. 2007AA10Z427)

摘要: 制备了高亲和力的伏马菌素B1(FB1)单克隆抗体,进而建立了谷物中FB1的直接竞争化学发光酶联免疫检测方法(dc-CLEIA)。采用碳化二亚胺法成功合成了FB1人工抗原,经杂交瘤技术获得分泌FB1特异性抗体的细胞株,高碘酸钠法酶标单克隆抗体后,建立了FB1的dc-CLEIA,本方法的IC50值为1.43 μg/L,线性范围为0.32-8.40 μg/L,检出限为0.13 μg/L,竞争反应时间仅需20 min。玉米样品加标回收率为100.2%-115.4%,相对标准偏差小于8.7%,谷物样品的检测结果与高效液相色谱法及商业化ELISA试剂盒检测结果相符。

English

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  • 收稿日期:  2012-02-29
  • 网络出版日期:  2012-06-04
通讯作者: 陈斌, bchen63@163.com
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