高效液相色谱法同时测定软饮料中20种食品添加剂

引用本文: 马康, 蒋孝雄, 赵敏, 蔡冶强. 高效液相色谱法同时测定软饮料中20种食品添加剂[J]. 分析化学, 2012, 40(11): 1661-1667. doi: 10.3724/SP.J.1096.2012.20176 shu

Citation: MA Kang, JIANG Xiao-Xiong, ZHAO Min, CAI Ye-Qiang. Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1661-1667. doi: 10.3724/SP.J.1096.2012.20176 shu

高效液相色谱法同时测定软饮料中20种食品添加剂

马康 ^{1,
,} ,
蒋孝雄 ^2, ,
赵敏 ^1, ,
蔡冶强 ^2,

通讯作者: 马康

基金项目:
本文系国家质检总局食品安全专项(No. ASPAQ1010) (No. ASPAQ1010)

中国计量科学研究院基础科研费(No. AKY1216)资助 (No. AKY1216)

摘要: 建立了高效液相色谱同时测量软饮料中20种食品添加剂(咖啡因、3种甜味剂、9种防腐剂和7种着色剂)的方法。确定的最佳分离条件为:C₁₈反相色谱柱(250 mm×4.6 mm×5 μm), 流动相为0.10 mol/L乙酸铵缓冲溶液(pH 7.2)和乙腈-甲醇混合有机相(10:90, V/V), 梯度洗脱, 二极管阵列检测器在190-700 nm监测。20种食品添加剂浓度在0.10-300 mg/L范围内具有良好的线性, 相关系数大于0.998; 20种食品添加剂的检出限在0.005-0.110 mg/L范围内;20种食品添加剂的加标回收率为91.4%-100.5%; 相对标准偏差计为0.6%-4.0%。本方法可应用于16个品牌的软饮料中食品添加剂的检测。

关键词:

English

Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector

1.
¹ Division of Chemical Metrology and Analytical Science, National Institute of Metrology of China, Beijing 100013, China;

Corresponding author: 马康

Received Date: 24 April 2012
Fund Project:
本文系国家质检总局食品安全专项(No. ASPAQ1010)

中国计量科学研究院基础科研费(No. AKY1216)资助

Abstract: An efficient and accurate analytical method was developed for the simultaneous determination of 20 synthetic food additives, including acesulfame potassium, benzoic acid, sorbic acid, sodium saccharin, tartrazine, amaranthus red, ponceau 4R, caffeine, sunset yellow, aspartame, allure red, brilliant blue, erythrosine, methyl paraben, ethyl paraben, isopropyl paraben, n-propyl paraben, isobutyl paraben, n-butyl paraben and n-heptyl paraben, by high performance liquid chromatography (HPLC) coupled with photodiode array detector(PDA). A C₁₈ stationary phase was used and the mobile phase contained an acetonitrile-methanol (10:90, V/V) mixture and 0.1 mol/L ammonium acetate buffer solution at pH 7.2. Successful separation was obtained for all the compounds within 50 min at 30℃ by using gradient elution. The absorbance of 20 compounds behaved linearly with their concentrations ranged from 0.10 mg/L to 300 mg/L with correlation coefficients more than 0.998. The limits of detection of these compounds were from 0.005 mg/L to 0.110 mg/L. The recoveries were from 91.4% to 100.5% at spiked levels of 10-100 mg/L, The precision (n=6) was range from 0.6% to 4.0%. The method has been used in the determination of 16 food additives in various drinks.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

高效液相色谱法同时测定软饮料中20种食品添加剂

通讯作者: 马康

English

Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector

Corresponding author: 马康

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高效液相色谱法同时测定软饮料中20种食品添加剂

通讯作者: 马康

English

Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector

Corresponding author: 马康

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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