基于“Click”反应耦合酶的高灵敏葡萄糖生物传感器的研究

引用本文: 李文娟, 于超, 王应雄, 杨竹. 基于“Click”反应耦合酶的高灵敏葡萄糖生物传感器的研究[J]. 分析化学, 2012, 40(11): 1642-1647. doi: 10.3724/SP.J.1096.2012.11370 shu

Citation: LI Wen-Juan, YU Chao, WANG Ying-Xiong, YANG Zhu. A Highly Sensitive Biosensor Based on a Versatile Approach for Immobilization of Glucose Oxidase via "Click" Reaction for Detection of Glucose[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1642-1647. doi: 10.3724/SP.J.1096.2012.11370 shu

基于“Click”反应耦合酶的高灵敏葡萄糖生物传感器的研究

通讯作者: 于超

基金项目:
本文系国家自然科学基金(No.21205146) (No.21205146)

重庆市自然科学基金(No. CSTC 2009BA5083)资助项目 (No. CSTC 2009BA5083)

摘要: 采用葡萄糖氧化酶(GOD)为模板, 利用"Click"反应固定, 构建了新型葡萄糖生物传感器。首先, 将碳纳米管叠氮化, 并将GOD炔基化;在Cu⁺的催化作用下, 两者发生"Click"反应;在Nafion的作用下固定在玻碳电极表面, 制得葡萄糖生物传感器。采用傅里叶红外光谱(FTIR)法对碳纳米管"Click"反应前后的性质进行了表征。采用循环伏安法和计时电流法考察了电极的电化学行为, 并对传感器的性能进行了详细研究。结果表明:在优化的实验条件下, 此电极对葡萄糖有明显的催化作用, 电流与葡萄糖的浓度在6.0×10^-7-1.4×10^-3 mol/L范围内呈现良好的线性关系, 检出限达2.0×10^-7 mol/L。此传感器具有良好的灵敏度、稳定性和重现性。对血清样品中的葡萄糖进行检测, 结果令人满意。

关键词:

English

A Highly Sensitive Biosensor Based on a Versatile Approach for Immobilization of Glucose Oxidase via "Click" Reaction for Detection of Glucose

1.
Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China

Corresponding author: 于超

Received Date: 05 February 2012
Fund Project:
本文系国家自然科学基金(No.21205146)

重庆市自然科学基金(No. CSTC 2009BA5083)资助项目

Abstract: A novel immobilized method, "click" reaction, was employed to capture glucose oxidase (GOD). First, azido-terminated carbon nanotubes (CNTs-N₃) were obtained by bifunctional azide molecule (amine-PEG-azide) and alkynyl-modified GOD (alkynyl-GOD) was also synthesized. Subsequently, CNTs-N₃ was reacted with alkynyl-GOD in a copper-catalyzed click reaction. Finally, the mixture was dipped onto the surface of glassy carbon electrode with the help of Nafion to construct a highly sensitive biosensor for the detection of glucose. The results of the both click reaction were monitored by IR spectroscopy, and the electrochemical performance of the modified electrode was studied by cyclic voltammetry and chronoamperometry. Under optimal conditions, the developed sensor exhibited fast response to glucose and the linear ranges were 6.0×10^-7-1.4×10^-3 mol/L with a detection limit of 2.0×10^-7 mol/L (at 3σ). The proposed biosensor exhibited high sensitivity, long-term stability, excellent reproducibility. In addition, the serum samples were analyzed by this biosensor with satisfactory results.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于“Click”反应耦合酶的高灵敏葡萄糖生物传感器的研究

通讯作者: 于超

English

A Highly Sensitive Biosensor Based on a Versatile Approach for Immobilization of Glucose Oxidase via "Click" Reaction for Detection of Glucose

Corresponding author: 于超

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于“Click”反应耦合酶的高灵敏葡萄糖生物传感器的研究

通讯作者: 于超

English

A Highly Sensitive Biosensor Based on a Versatile Approach for Immobilization of Glucose Oxidase via "Click" Reaction for Detection of Glucose

Corresponding author: 于超

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs