锰掺杂硫化锌量子点室温磷光检测铅离子

引用本文: 陈娟, 孙洁芳, 郭磊, 谢剑炜, 孙国祥. 锰掺杂硫化锌量子点室温磷光检测铅离子[J]. 分析化学, 2012, 40(11): 1680-1685. doi: 10.3724/SP.J.1096.2011.10225 shu

Citation: CHEN Juan, SUN Jie-Fang, GUO Lei, XIE Jian-Wei, SUN Guo-Xiang. Determination of Lead Ion Based on Quenching of Room Temperature Phosphorescence of Mn-doped ZnS Quantum Dots[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1680-1685. doi: 10.3724/SP.J.1096.2011.10225 shu

锰掺杂硫化锌量子点室温磷光检测铅离子

陈娟 ^1,2, ,
孙洁芳 ^1, ,
郭磊 ^1, ,
谢剑炜 ^{1,
,} ,
孙国祥 ^2,

通讯作者: 谢剑炜

基金项目:
本文系"重大新药创制"国家科技重大专项(Nos.2009ZX09301-002, 2010ZXJ0900X-003-002)资助项目 (Nos.2009ZX09301-002, 2010ZXJ0900X-003-002)

摘要: 采用锰掺杂硫化锌量子点室温磷光(RTP)探针, 基于Pb²⁺对其磷光的猝灭现象进行选择性测定, 考察了量子点溶液浓度、缓冲液体系及浓度、反应时间、干扰物质等多种因素影响, 在最优条件下, 即25 μmol/L量子点溶液在Tris-HCl(10 mmol/L, pH 7.4)缓冲液中, 反应时间为2 min时, 检测Pb²⁺的线性范围为0.25-1000 μmol/L, 检出限为0.04 μmol/L, 方法的精密度和准确度良好。本方法简单、快速、灵敏度和选择性较好, 线性范围较宽, 可用于96孔板进行高通量检测, 并成功应用于生物样品中Pb²⁺的选择性检测, 在稀释5倍人血浆中检测Pb²⁺的线性范围为5-1000 μmol/L, 检出限为0.48 μmol/L。

关键词:

English

Determination of Lead Ion Based on Quenching of Room Temperature Phosphorescence of Mn-doped ZnS Quantum Dots

1.
¹ Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China;

Corresponding author: 谢剑炜

Received Date: 11 January 2012
Fund Project:
本文系"重大新药创制"国家科技重大专项(Nos.2009ZX09301-002, 2010ZXJ0900X-003-002)资助项目

Abstract: Room temperature phosphorescence (RTP) property of Mn-doped ZnS quantum dots (QDs) was explored to develop a novel method for selective detection of lead cation. The influence factors such as buffer kinds and their concentration, the concentration of Mn-doped ZnS QDs solution, reaction time and coexisting substances were studied. It was found that under optimized conditions, i.e. 10 mmol/L Tris-HCl buffer at pH 7.4 and 2 min reaction time, the relative RTP intensity of the Mn-doped ZnS QDs was quenched linearly by lead cation in the concentration range from 0.25 to 1000 μmol/L, and the limit of detection was 0.04 μmol/L (8.3 μg Pb/L). This method was also applied to measure lead cation in biosamples using a high-throughput 96 well plate with a wide linear range, simpleness, rapidness, high sensitivity and selectivity, and cost effectiveness. The RTP was quenched linearly by lead cation in human plasma in the concentration range from 5 to 1000 μmol/L, and the limit of detection was 0.48 μmol/L (Pb 99 μg/L), respectively.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

锰掺杂硫化锌量子点室温磷光检测铅离子

通讯作者: 谢剑炜

English

Determination of Lead Ion Based on Quenching of Room Temperature Phosphorescence of Mn-doped ZnS Quantum Dots

Corresponding author: 谢剑炜

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

锰掺杂硫化锌量子点室温磷光检测铅离子

通讯作者: 谢剑炜

English

Determination of Lead Ion Based on Quenching of Room Temperature Phosphorescence of Mn-doped ZnS Quantum Dots

Corresponding author: 谢剑炜

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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