Abstract: Preparation of huperzine A molecularly imprinted polymers(MIPs) using silica as sacrificial material was carried out for the first time by using huperzine A as template molecule,methyl acrylic acid as functional monomer,divinyl benzene as cross-linker and 2'-2-azobis-isobutyronitrile as initiator,respectively. The MIPs were characterized by infrared spectroscopy(IR),scanning electron microscopy(SEM) and thermogravimetic analysis. Static method was employed to measure adsorption isotherm and Scatchard analysis was used to investigate on site distribution on the surface of MIPs. Results indicated that such MIPs exhibited a good selective adsorption performance with a selectivity coefficient of 1.399 toward the template. Two types of adsorption sites were mainly found in the matrix of the imprinted polymers with dissociation constants and apparent maximum binding capacity of 0.169 g/L and 0.832 mg/g for the low affinity sites,and 0.776 g/L and 0.213 mg/g for the high affinity ones. Additionally,a good enrichment capability toward huperzine A was also observed when this MIPs was used for solid phase micro-extraction of target compound from crude extract solution of Serrate Clubmoss. A recovery of 93.5% for the product could be achieved.