基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

引用本文: 阮栎婷, 何绍英, 赵婉, 曹红帅, 徐志爱, 张文. 基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究[J]. 分析化学, 2022, 50(9): 1319-1327. doi: 10.19756/j.issn.0253-3820.221099 shu

Citation: RUAN Li-Ting, HE Shao-Ying, ZHAO Wan, CAO Hong-Shuai, XU Zhi-Ai, ZHANG Wen. Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1319-1327. doi: 10.19756/j.issn.0253-3820.221099 shu

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

华东师范大学化学与分子工程学院, 上海 200241

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

基金项目:
国家自然科学基金项目（No.22174047）资助。

摘要: 以精氨酸(Arg)取代乙二胺四乙酸(EDTA)作为稳定剂,采用水热法合成了钒酸铈(CeVO₄)纳米酶。稳定剂的改变使得CeVO₄纳米材料的形貌从纳米棒转变为纳米粒子,同时,CeVO₄中Ce^Ⅲ和Ce^Ⅳ比值改变使得纳米酶活性由较强的类超氧化物歧化酶(SOD)转变成较强的类过氧化物酶(POD)。在3,3',5,5'-四甲基联苯胺(TMB)和过氧化氢(H₂O₂)反应体系中,CeVO₄纳米粒子催化H₂O₂产生羟基自由基(·OH),·OH氧化TMB,使溶液由无色变为蓝色,证明其具有较强的类POD活性。基于CeVO₄纳米酶的类POD活性,建立了检测H₂O₂和葡萄糖的比色传感平台,H₂O₂和葡萄糖响应的线性范围分别为10~1500 μmol/L和10~120 μmol/L,检出限(3σ)分别为1.1 μmol/L和3.3 μmol/L。将本方法应用于实际样品中总抗氧化能力(TAC)分析,结果令人满意。

关键词:

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

Received Date: 24 February 2022
Revised Date: 13 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.22174047).

Abstract: The cerium vanadate (CeVO₄)-based nanozyme was synthesized through a hydrothermal synthesis method with arginine as the stabilizer. Due to the change of stabilizer, the ratio of Ce^Ⅲ to Ce^Ⅳ in CeVO₄ changed and thus allowed that the nanozyme showed nanoparticle morphology, which was different from that of nanorod prepared with EDTA as stabilizer. In the reaction system of 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂), the CeVO₄-based nanozyme first catalyzed H₂O₂ to produce hydroxyl radical (·OH), which could oxidize TMB and thus resulted in a colour change of solution from colourless to blue, proving that CeVO₄ prepared with arginine had strong peroxidase-like activity and the generation of ·OH accounted for the outstanding peroxidase-like properties. Based on these features, a colorimetric method was developed for detecting H₂O₂, glucose and antioxidants. This study not only provided a new direction for the research of CeVO₄-based nanozyme, but also developed a fast and simple method for total antioxidant capacity (TAC) assay.

Key words:

1. [1]
  BENKOVIC S J, HAMMES-SCHIFFER S. Science, 2003, 301(5637):1196-1202.BENKOVIC S J, HAMMES-SCHIFFER S. Science, 2003, 301(5637):1196-1202.
2. [2]
  BEAUDRY A A, JOYCE G F. Science, 1992, 257(5070):635-641.BEAUDRY A A, JOYCE G F. Science, 1992, 257(5070):635-641.
3. [3]
  WEI H, GAO L, FAN K, LIU J, HE J, QU X, DONG S, WANG E, YAN J. Nano Today, 2021, 40:101269.WEI H, GAO L, FAN K, LIU J, HE J, QU X, DONG S, WANG E, YAN J. Nano Today, 2021, 40:101269.
4. [4]
  FAN K, CAO C, PAN Y, LU D, YANG D L, FENG J, SONG L N, LIANG M M, YAN X Y. Nat. Nanotechnol., 2012, 7(7):459-464.FAN K, CAO C, PAN Y, LU D, YANG D L, FENG J, SONG L N, LIANG M M, YAN X Y. Nat. Nanotechnol., 2012, 7(7):459-464.
5. [5]
  HE W, ZHOU Y, WAMER W G, HU X, WU X, ZHENG Z. Biomaterials, 2013, 34(3):765-773.HE W, ZHOU Y, WAMER W G, HU X, WU X, ZHENG Z. Biomaterials, 2013, 34(3):765-773.
6. [6]
  JIN L, DONG Y, WU X, CAO G, WANG G. Anal. Chem., 2015, 87(20):10429-10436.JIN L, DONG Y, WU X, CAO G, WANG G. Anal. Chem., 2015, 87(20):10429-10436.
7. [7]
  LIU Y, ZHOU M, CAO W, WANG X, WANG Q, LI S, WEI H. Anal. Chem., 2019, 91(13):8170-8175.LIU Y, ZHOU M, CAO W, WANG X, WANG Q, LI S, WEI H. Anal. Chem., 2019, 91(13):8170-8175.
8. [8]
  MANEA F, HOUILLON F B, PASQUATO L, SCRIMIN P. Angew. Chem., Int. Ed., 2004, 43(45):6165-6169.MANEA F, HOUILLON F B, PASQUATO L, SCRIMIN P. Angew. Chem., Int. Ed., 2004, 43(45):6165-6169.
9. [9]
  NATALIO F, ANDRE R, HARTOG A F, STOLL B, JOCHUM K P, WEVER R, TREMEL W. Nat. Nanotechnol., 2012, 7(8):530-535.NATALIO F, ANDRE R, HARTOG A F, STOLL B, JOCHUM K P, WEVER R, TREMEL W. Nat. Nanotechnol., 2012, 7(8):530-535.
10. [10]
  SOH M, KANG D W, JEONG H G, KIM D, KIM D Y, YANG W, SONG C, BAIK S, CHOI I Y, KI S K. Angew. Chem., Int. Ed., 2017, 56(38):11399-11403.SOH M, KANG D W, JEONG H G, KIM D, KIM D Y, YANG W, SONG C, BAIK S, CHOI I Y, KI S K. Angew. Chem., Int. Ed., 2017, 56(38):11399-11403.
11. [11]
  SONG Y, QU K, ZHAO C, REN J, QU X. Adv. Mater., 2010, 22(19):2206-2210.SONG Y, QU K, ZHAO C, REN J, QU X. Adv. Mater., 2010, 22(19):2206-2210.
12. [12]
  SUN H, ZHOU Y, REN J, QU X. Angew. Chem., Int. Ed., 2018, 57(30):9224-9237.SUN H, ZHOU Y, REN J, QU X. Angew. Chem., Int. Ed., 2018, 57(30):9224-9237.
13. [13]
  TARNUZZER R W, COLON J, PATIL S, SEAL S. Nano Lett., 2005, 5(12):2573-2577.TARNUZZER R W, COLON J, PATIL S, SEAL S. Nano Lett., 2005, 5(12):2573-2577.
14. [14]
  WANG X, GAO X, QIN L, WANG C, SONG L, ZHOU Y, ZHU G, CAO W, LIN S, ZHOU L. Nat. Commun., 2019, 10:704.WANG X, GAO X, QIN L, WANG C, SONG L, ZHOU Y, ZHU G, CAO W, LIN S, ZHOU L. Nat. Commun., 2019, 10:704.
15. [15]
  ZHANG W, HU S, YIN J, HE W, LU W, MA M, GU N, ZHANG Y. J. Am. Chem. Soc., 2016, 138(18):5860-5865.ZHANG W, HU S, YIN J, HE W, LU W, MA M, GU N, ZHANG Y. J. Am. Chem. Soc., 2016, 138(18):5860-5865.
16. [16]
  DUAN D, FAN K, ZHANG D, TAN S, LIANG M, LIU Y, ZHANG J, ZHANG P, LIU W, QIU X. Biosens. Bioelectron., 2015, 74:134-141.DUAN D, FAN K, ZHANG D, TAN S, LIANG M, LIU Y, ZHANG J, ZHANG P, LIU W, QIU X. Biosens. Bioelectron., 2015, 74:134-141.
17. [17]
  ZHU Y, WU J, HAN L, WANG X, LI W, GUO H, WEI H. Anal. Chem., 2020, 92(11):7444-7452.ZHU Y, WU J, HAN L, WANG X, LI W, GUO H, WEI H. Anal. Chem., 2020, 92(11):7444-7452.
18. [18]
  LIN J, WANG Q, WANG X, ZHU Y, ZHOU X, WEI H. Analyst, 2020, 145:3916-3921.LIN J, WANG Q, WANG X, ZHU Y, ZHOU X, WEI H. Analyst, 2020, 145:3916-3921.
19. [19]
  WEERATHUNGE P, RAMANATHAN R, SHARMA T K, BANSAL V. Anal. Chem., 2014, 86(24):11937-11941.WEERATHUNGE P, RAMANATHAN R, SHARMA T K, BANSAL V. Anal. Chem., 2014, 86(24):11937-11941.
20. [20]
  WU G, HE S, PENG H, DENG H, LIU A, LIN H, XIA H, CHEN W. Anal. Chem., 2014, 86(21):10955-10960.WU G, HE S, PENG H, DENG H, LIU A, LIN H, XIA H, CHEN W. Anal. Chem., 2014, 86(21):10955-10960.
21. [21]
  CHEN J, SUN Y, LI H, HU X. Talanta, 2018, 189:254-261.CHEN J, SUN Y, LI H, HU X. Talanta, 2018, 189:254-261.
22. [22]
  ORTIZ-GOMEZ I, SALINAS-CASTILLO A, GARCIA A G, ALVAREZ-BERMEJO J A, DE ORBE-PAYA I, RODRIGUEZ-DIEGUEZ A, CAPITAN-VALLVEY L F. Microchim. Acta, 2018, 185:47.ORTIZ-GOMEZ I, SALINAS-CASTILLO A, GARCIA A G, ALVAREZ-BERMEJO J A, DE ORBE-PAYA I, RODRIGUEZ-DIEGUEZ A, CAPITAN-VALLVEY L F. Microchim. Acta, 2018, 185:47.
23. [23]
  XI J, ZHU C, WANG Y, ZHANG Q, FAN L. RSC Adv., 2019, 9:16509.XI J, ZHU C, WANG Y, ZHANG Q, FAN L. RSC Adv., 2019, 9:16509.
24. [24]
  JIAO A, XU L, TIAN Y, CUI Q, LIU X, CHEN M. Talanta, 2021, 225:121990.JIAO A, XU L, TIAN Y, CUI Q, LIU X, CHEN M. Talanta, 2021, 225:121990.
25. [25]
  SHARMA T S K, HWA K, SANTHAN A, GANGULY A. Sens. Actuators, B, 2021, 331:129413.SHARMA T S K, HWA K, SANTHAN A, GANGULY A. Sens. Actuators, B, 2021, 331:129413.
26. [26]
  KOKULNATHAN T, KARTHIK R, CHEN S, KUMAR J V, SAKTHINATHANA S. Microchim. Acta, 2019, 186:579.KOKULNATHAN T, KARTHIK R, CHEN S, KUMAR J V, SAKTHINATHANA S. Microchim. Acta, 2019, 186:579.
27. [27]
  DENISOVA L, CHUMILINA L, KARGIN Y F, DENISOV V. Inorg. Mater., 2016, 52:44-47.DENISOVA L, CHUMILINA L, KARGIN Y F, DENISOV V. Inorg. Mater., 2016, 52:44-47.
28. [28]
  LIU H, YANG Z. Mater. Sci. Eng., B, 2021, 269:115159.LIU H, YANG Z. Mater. Sci. Eng., B, 2021, 269:115159.
29. [29]
  JIN R, LIU C, SUN L, ZHANG Z, CHEN G. ChemElectroChem, 2016, 3(4):644-649.JIN R, LIU C, SUN L, ZHANG Z, CHEN G. ChemElectroChem, 2016, 3(4):644-649.
30. [30]
  SINGH N, MUGESH G. Angew. Chem., Int. Ed., 2019, 58(23):7797-7801.SINGH N, MUGESH G. Angew. Chem., Int. Ed., 2019, 58(23):7797-7801.
31. [31]
  YANG W, LING B, HU B, YIN H, MAO J, PATRICK J W. Angew. Chem., Int. Ed., 2020, 59(1):2-12.YANG W, LING B, HU B, YIN H, MAO J, PATRICK J W. Angew. Chem., Int. Ed., 2020, 59(1):2-12.
32. [32]
  SZYMANSKI C J, MUNUSAMY P, MIHAI C, XIE Y, HUA D, GILLES M K, ORRA G. Biomaterials, 2015, 62:147-154.SZYMANSKI C J, MUNUSAMY P, MIHAI C, XIE Y, HUA D, GILLES M K, ORRA G. Biomaterials, 2015, 62:147-154.
33. [33]
  DONG H, FAN Y, ZHANG W, GU N, ZHANG Y. Bioconjugate Chem., 2019, 30(5):1273-1296.DONG H, FAN Y, ZHANG W, GU N, ZHANG Y. Bioconjugate Chem., 2019, 30(5):1273-1296.
34. [34]
  ZHOU Y, SUN H, XU H, MATYSIAK S, QU X. Angew. Chem., Int. Ed., 2018, 57(51):16791-16795.ZHOU Y, SUN H, XU H, MATYSIAK S, QU X. Angew. Chem., Int. Ed., 2018, 57(51):16791-16795.
35. [35]
  CHEN J, PEZZATO C, SCRIMIN P, PRINS L. Chem.-Eur. J., 2016, 22:7028-7032.CHEN J, PEZZATO C, SCRIMIN P, PRINS L. Chem.-Eur. J., 2016, 22:7028-7032.
36. [36]
  LIU J, WANG L, SUN X, ZHU X. Angew. Chem., Int. Ed., 2010, 49(20):3492-3495.LIU J, WANG L, SUN X, ZHU X. Angew. Chem., Int. Ed., 2010, 49(20):3492-3495.
37. [37]
  YANG H, ZHA J, ZHANG P, QIN Y, CHEN T, YE F. Sens. Actuators, B, 2017, 247:469-478.YANG H, ZHA J, ZHANG P, QIN Y, CHEN T, YE F. Sens. Actuators, B, 2017, 247:469-478.
38. [38]
  ZHU L, LI Q, LI J, LIU X, MENG J, CAO X. Nanoparticle Res., 2007, 99:261-268.ZHU L, LI Q, LI J, LIU X, MENG J, CAO X. Nanoparticle Res., 2007, 99:261-268.
39. [39]
  HUANG Z, HE W, SHEN H, HAN G, WANG H, SU P, SONG J, YANG Y. Talanta, 2021, 230:122337.HUANG Z, HE W, SHEN H, HAN G, WANG H, SU P, SONG J, YANG Y. Talanta, 2021, 230:122337.
40. [40]
  GAO L, ZHANG J, NIE L, ZHANG J, ZHANG Y, GU N, WANG T, FENG J, YANG D, PERRETT S, YAN X. Nat. Nanotechnol., 2007, 2(9):577-583.GAO L, ZHANG J, NIE L, ZHANG J, ZHANG Y, GU N, WANG T, FENG J, YANG D, PERRETT S, YAN X. Nat. Nanotechnol., 2007, 2(9):577-583.
41. [41]
  ZHANG Y, ZHOU Z, WEN F, TAN J, PENG T, LUO B, WANG H, YIN S. Sens. Actuators, B, 2018, 275:155-162.ZHANG Y, ZHOU Z, WEN F, TAN J, PENG T, LUO B, WANG H, YIN S. Sens. Actuators, B, 2018, 275:155-162.
42. [42]
  GE C, WU R, CHONG G, FANG X, JIANG X, PAN C, CHEN C, YIN J. Adv. Funct. Mater., 2018, 28:1801484.GE C, WU R, CHONG G, FANG X, JIANG X, PAN C, CHEN C, YIN J. Adv. Funct. Mater., 2018, 28:1801484.
43. [43]
  SANKARARAMAKRISHINAN N, SHANKHWAR A, CHAUHAN D. Chemosphere, 2019, 228:390-397.SANKARARAMAKRISHINAN N, SHANKHWAR A, CHAUHAN D. Chemosphere, 2019, 228:390-397.
44. [44]
  ZHUO S, FANG J, ZHU C, DU J. Anal. Bioanal. Chem., 2020, 412:963-972.ZHUO S, FANG J, ZHU C, DU J. Anal. Bioanal. Chem., 2020, 412:963-972.
45. [45]
  SHI W, ZHANG X, HE S, HUANG Y. Chem. Commun., 2011, 47:10785-10787.SHI W, ZHANG X, HE S, HUANG Y. Chem. Commun., 2011, 47:10785-10787.
46. [46]
  SON S E, GUPTA P K, HUR W, LEE H B, PARK Y, PARK J, KIM S N, SEONG G H. ACS Appl. Nano Mater., 2021, 4(8):8282-8291.SON S E, GUPTA P K, HUR W, LEE H B, PARK Y, PARK J, KIM S N, SEONG G H. ACS Appl. Nano Mater., 2021, 4(8):8282-8291.
47. [47]
  ZENG Y, LI Y, TAN X, GONG J, WANG Z, AN Y, WANG Z, LI H. ACS Appl. Mater. Interfaces, 2021, 13(31):36816-36823.ZENG Y, LI Y, TAN X, GONG J, WANG Z, AN Y, WANG Z, LI H. ACS Appl. Mater. Interfaces, 2021, 13(31):36816-36823.

扫一扫看文章

计量

PDF下载量: 12
文章访问数: 545
HTML全文浏览量: 61

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs