基于量子级联激光器中红外光谱技术的幽门螺旋杆菌呼气诊断的可行性研究

引用本文: 黄慧, 周亦辰, 彭宇, 李雪, 张晔, 朱地. 基于量子级联激光器中红外光谱技术的幽门螺旋杆菌呼气诊断的可行性研究[J]. 分析化学, 2022, 50(9): 1328-1335. doi: 10.19756/j.issn.0253-3820.221080 shu

Citation: HUANG Hui, ZHOU Yi-Chen, PENG Yu, LI Xue, ZHANG Ye, ZHU Di. Feasibility Study of Breath Diagnosis in Helicobacter Pylori Based on Quantum Cascade Laser Mid-Infrared Spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1328-1335. doi: 10.19756/j.issn.0253-3820.221080 shu

基于量子级联激光器中红外光谱技术的幽门螺旋杆菌呼气诊断的可行性研究

黄慧 ^1, ,
周亦辰 ^2, ,
彭宇 ^3, ,
李雪 ^2, ,
张晔 ^{1,
,} ,
朱地 ^{4,
,}

1.
暨南大学附属第一医院口腔科, 暨南大学口腔医学院, 广州 510632;
2.
暨南大学质谱仪器与大气环境研究所, 广东省大气污染在线源解析系统工程技术研究中心, 广州 510632;
3.
苏州冠德能源科技有限公司, 苏州 215000;
4.
齐鲁工业大学(山东省科学院)能源研究所, 济南 250014

通讯作者: 张晔,E-mail:yzhang@jnu.edu.cn; 朱地,E-mail:zhud@sderi.cn

基金项目:
生物质应用量检测技术与装备开发项目（No.2019GXRC046）和国家自然科学基金项目（No.22122603）资助。

摘要: 幽门螺旋杆菌(Hp)与胃、十二指肠疾病密切相关,被证明是胃癌的Ⅰ类致癌因子。碳13尿素呼气试验(¹³C-UBT)因具有准确、简单、无放射性和非侵入性的优势,已成为检测Hp感染的初步诊断和治愈确认的首选测试方法。但是,因测试仪器昂贵且¹³C尿素试剂全球产量有限导致其价格不菲,使得¹³C-UBT仍是一种成本较高的Hp感染检测方法。为实现¹³C-UBT对Hp的高灵敏、低成本的实时检测,本研究探究了高分辨率、高灵敏度的中红外量子级联激光光谱技术替代传统基于质谱和近红外光谱技术作为Hp检测仪器的可行性。选择纳入Hp感染阳性、阴性、治疗中的受试者,考察了呼气采样方式(口呼气和鼻呼气)、¹³C尿素服用剂量(5、10、20和75 mg)对Hp感染诊断结果的影响。结果表明,中红外量子级联激光光谱技术具有实时原位测量、准确度高的特点,最大测量误差约为0.1‰~0.3‰。呼气采样方式结果表明,口呼气和鼻呼气两种采样方式得到的测量值一致(p>0.05),表明鼻呼气有望替代口呼气为Hp呼气检测采集样本,进而减少口腔菌群对Hp检测结果的影响。不同¹³C尿素服用剂量的实验结果表明,服用5 mg ¹³C尿素即可获得准确的诊断结果,与临床上现行¹³C尿素剂量(75~100 mg)相比,大幅度节省了试剂成本。本研究检测了基于量子级联激光器的中红外光谱分析仪的测量准确度,考察了其用于检测Hp感染的呼气诊断的可行性,优化了Hp呼气试验的采样方式和适宜的¹³C尿素剂量,为后续大规模样本检测提供了必要的实验基础。

关键词:

English

Feasibility Study of Breath Diagnosis in Helicobacter Pylori Based on Quantum Cascade Laser Mid-Infrared Spectroscopy

HUANG Hui ^1, ,
ZHOU Yi-Chen ^2, ,
PENG Yu ^3, ,
LI Xue ^2, ,
ZHANG Ye ^{1,
,} ,
ZHU Di ^{4,
,}

1.
College of Stomatology, Department of Stomatology, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510632, China;
2.
Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
3.
Suzhou Guande Energy Tech LLC, Suzhou 215000, China;
4.
Energy Research Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China

Corresponding author: ZHANG Ye, ; ZHU Di,

Received Date: 18 February 2022
Revised Date: 12 May 2022
Fund Project:
Supported by the Biomass Application Quantity Detection Technology and Equipment Development Project (No.2019GXRC046) and the National Natural Science Foundation of China (No.22122603).

Abstract: Helicobacter pylori (Hp), which has proven to be a class I carcinogen of gastric cancer, can cause various gastrointestinal diseases including stomach and duodenum. The carbon-13 urea breath test (¹³C-UBT) is considered to be the chief choice for the initial diagnosis and confirmation of Hp infection due to its advantages such as accuracy, simplicity, non-radioactivity, and non-invasiveness. However, ¹³C-UBT remains to be an expensive method for detecting Hp infection owing to the high cost of testing equipment and the limited global production of ¹³C urea. To realize the real-time detection of Hp with high sensitivity and low cost by ¹³C-UBT, this study explored the feasibility of exploiting high-resolution and high-sensitivity mid-infrared quantum cascade laser spectroscopy to replace the traditional detection instruments based on mass spectrometry and near-infrared spectroscopy as Hp detection instruments. The Hp infection-positive, negative, and treated subjects were included, and the influences of sampling methods (mouth and nose) and ¹³C urea dosages (5, 10, 20, and 75 mg) on the diagnosis of Hp infection were investigated. The results showed that the mid-infrared quantum cascade laser spectroscopy possessed the characteristics of real-time in-situ measurement and high levels of accuracy, and the maximum measurement error was about 0.1‰-0.3‰. The results of the breath sampling methods indicated that the measured values obtained from the sampling methods of mouth and nose kept consistent (p>0.05), proving that nose air would be highly desirable as an alternative to mouth air to reduce the interference of oral flora on detection results when collecting breath samples for Hp detection. The test results of different dosages demonstrated that accurate diagnosis results could be obtained by administrating 5 mg of ¹³C urea, and the reagent costs could be crucially saved when compared with the current clinical ¹³C urea dose (75-100 mg). In conclusion, this study examined the measurement accuracy of the infrared spectrum instrument based on the quantum cascade lasers, investigated its feasibility for detecting Hp infection of the breath diagnosis, as well as optimized the breath sampling methods and suitable ¹³C urea dosages, which provided necessary experimental basis for subsequent mass samples detection.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于量子级联激光器中红外光谱技术的幽门螺旋杆菌呼气诊断的可行性研究

通讯作者: 张晔,E-mail:yzhang@jnu.edu.cn; 朱地,E-mail:zhud@sderi.cn

English

Feasibility Study of Breath Diagnosis in Helicobacter Pylori Based on Quantum Cascade Laser Mid-Infrared Spectroscopy

Corresponding author: ZHANG Ye, ; ZHU Di,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于量子级联激光器中红外光谱技术的幽门螺旋杆菌呼气诊断的可行性研究

通讯作者: 张晔,E-mail:yzhang@jnu.edu.cn; 朱地,E-mail:zhud@sderi.cn

English

Feasibility Study of Breath Diagnosis in Helicobacter Pylori Based on Quantum Cascade Laser Mid-Infrared Spectroscopy

Corresponding author: ZHANG Ye, ; ZHU Di,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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