基于三叉型离子交换膜的带电粒子微流控富集芯片的研究

引用本文: 姜金华, 从奥博, 王月坤, 边超, 李洋, 佟建华. 基于三叉型离子交换膜的带电粒子微流控富集芯片的研究[J]. 分析化学, 2022, 50(4): 585-592. doi: 10.19756/j.issn.0253-3820.221077 shu

Citation: JIANG Jin-hua, CONG Ao-bo, WANG Yue-kun, BIAN Chao, LI Yang, TONG Jian-hua. Research on Charged Particle Microfluidic Enrichment Chip Based on Ion Exchange Membrane with Trigeminal Structure[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 585-592. doi: 10.19756/j.issn.0253-3820.221077 shu

基于三叉型离子交换膜的带电粒子微流控富集芯片的研究

姜金华 ^1,2, ,
从奥博 ^1,2, ,
王月坤 ^1,2, ,
边超 ^1, ,
李洋 ^1, ,
佟建华 ^{1,
,}

1.
中国科学院空天信息创新研究院传感技术国家重点实验室, 北京 100190;2 中国科学院大学电子电气与通信工程学院, 北京 100049

通讯作者: 佟建华,E-mail:jhtong@mail.ie.ac.cn

基金项目:
国家重点研发计划项目(No.2020YFB2009001)

国家自然科学基金项目(Nos.61771451,62071455)资助

摘要: 预富集是实现痕量生化物质检测的有效手段。本研究基于离子浓差极化(ICP)原理,研制了一种用于水环境中带电粒子的富集与检测的基于三叉型离子交换膜的微流控富集芯片。此芯片采用3D打印及微纳加工技术制备,通过控制富集区和缓冲区的电势差控制电渗力与排斥力,以获得较稳定的耗尽区和富集区。在富集实验中,以带负电荷的荧光素钠作为富集对象,探索了驱动电压及富集时间对富集倍率的影响,在0.2~3.0μmol/L的浓度范围内验证了富集效果。实验结果表明,本研究设计的基于离子交换膜的三叉型结构可有效避免富集区与缓冲区溶液的渗透,大幅提高了富集倍数;此芯片对不同浓度的荧光素钠的富集倍率基本一致,最高可达到102倍,具有良好的富集阴离子的能力。

关键词:

English

Research on Charged Particle Microfluidic Enrichment Chip Based on Ion Exchange Membrane with Trigeminal Structure

1.
State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China;2 School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: TONG Jian-hua, jhtong@mail.ie.ac.cn

Received Date: 16 February 2022
Revised Date: 13 March 2022
Fund Project:
Supported by the National Key Research and Development Project of China(No. 2020YFB2009001)

the National Natural Science Foundation of China(Nos. 61771451, 62071455)

Abstract: Preconcentration is an effective method to detect trace biochemical substances. In this work, a microfluidic enrichment chip based on ion exchange membrane with trident structure was developed for both the detection of charged particles in water environment and the enrichment of anions based on the principle of ion concentration polarization. The chip was fabricated by 3D printing and micro-nano processing technology. By controlling the potential difference between enrichment zone and buffer zone, the electroosmotic force and repulsion force could be controlled to obtain a stable depletion zone and enrichment zone. In the enrichment experiment, when the negatively charged sodium fluorescein was used as the enrichment object, the factors affecting the enrichment factor such as voltage and enrichment time were explored, and the enrichment effect was verified in the concentration range of 0.2-3.0 μmol/L. The study showed that the trident ion exchange membrane could effectively avoid the permeation of the solution in the enrichment zone and buffer zone as experimental results, which greatly improved the enrichment factor. The enrichment factor of different concentrations of sodium fluorescein on this chip was close, and the highest was 102 times, which meant that the chip had the ability of enriching anions.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于三叉型离子交换膜的带电粒子微流控富集芯片的研究

通讯作者: 佟建华,E-mail:jhtong@mail.ie.ac.cn

English

Research on Charged Particle Microfluidic Enrichment Chip Based on Ion Exchange Membrane with Trigeminal Structure

Corresponding author: TONG Jian-hua, jhtong@mail.ie.ac.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于三叉型离子交换膜的带电粒子微流控富集芯片的研究

通讯作者: 佟建华,E-mail:jhtong@mail.ie.ac.cn

English

Research on Charged Particle Microfluidic Enrichment Chip Based on Ion Exchange Membrane with Trigeminal Structure

Corresponding author: TONG Jian-hua, jhtong@mail.ie.ac.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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