制备MoS<sub>2</sub>修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测

引用本文: 马如龙, 郝茜, 梁梦佳, 余延恺, 牛娜, 陈立钢. 制备MoS₂修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测[J]. 分析化学, 2022, 50(4): 545-553. doi: 10.19756/j.issn.0253-3820.221035 shu

Citation: MA Ru-long, HAO Qian, LIANG Meng-jia, YU Yan-kai, NIU Na, CHEN Li-gang. Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 545-553. doi: 10.19756/j.issn.0253-3820.221035 shu

制备MoS₂修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测

东北林业大学化学化工与资源利用学院, 哈尔滨 150040

通讯作者: 牛娜,E-mail:niuna@nefu.edu.cn; 陈立钢,E-mail:ligangchen2008@163.com

基金项目:
黑龙江省自然科学基金项目(No.B2008001)

国家自然科学基金项目(No.21401019)

东北林业大学省级大学生创新训练项目(No.202110225341)资助

摘要: 采用水热合成法制备了MoS₂修饰的秸秆碳点纳米酶(Mo,S-CDs)用于人尿液中尿酸含量的检测,发挥了秸秆自身的结构优势,并实现了生物质废弃物的回收利用。对Mo,S-CDs的表征结果表明,Mo元素和S元素成功地修饰在秸秆碳点结构中,Mo,S-CDs为球形,表面有丰富的官能团,保障了其可较好地分散于水介质中,晶体层面为无定形碳结构。通过测试,Mo,S-CDs具有类天然过氧化物酶的活性,催化效率高,可在含有H₂O₂的体系中快速催化3,3’,5,5’-四甲基联苯胺氧化生成显色产物,显示出蓝色的特征响应颜色信号,在652 nm处有最大吸收。利用尿酸在尿酸酶的催化下生成H₂O₂的反应,基于Mo,S-CDs对H₂O₂的传感能力构建了尿酸酶-Mo,S-CDs的级联催化传感体系,并将其应用于人尿液中尿酸含量的检测。此传感体系用于检测尿酸,在5~100μmol/L浓度范围内具有良好的线性关系,并具有较低的检出限(1.8μmol/L)、较高的回收率(96.8%~106.1%)和较低的相对标准偏差(<5%)。研究结果表明,相比于天然过氧化物酶,Mo,S-CDs具有适用温度范围宽、稳定性好等优势,可广泛应用于生命分析等领域。

关键词:

English

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China

Corresponding author: NIU Na, ; CHEN Li-gang,

Received Date: 19 January 2022
Revised Date: 28 February 2022
Fund Project:
the Natural Science Foundation of Heilongjiang Province,China(No. B2008001)

Supported by the National Natural Science Foundation of China(No. 21401019)

the Provincial College Students’ Innovation Training Program of Northeast Forestry University(No. 202110225341)

Abstract: The MoS₂-modified straw carbon dots nanozyme(Mo, S-CDs) was prepared by hydrothermal synthesis for detection of content of uric acid in human urine. This work fully exploited the inherent advantages of straw structure and realized the recycling of biomass waste. The characterization of Mo, S-CDs showed that Mo and S elements were successfully modified into the structure of straw carbon dots, and the Mo, S-CDs were spherical with abundant functional groups on the surface to ensure their good dispersion in aqueous medium and amorphous carbon structure at crystal level. The Mo, S-CDs had the activity of mimetic peroxidase and showed high catalytic efficiency. They were able to rapidly catalyze the oxidation of 3, 3', 5, 5'-tetramethylbenzidine in a system containing H₂O₂ to generate chromogenic product, which visually showed a characteristic blue color signal in response and reached a peak absorbance at 652 nm.The cascade catalytic sensing system of uric acid-Mo, S-CDs was constructed based on the sensing ability of Mo, S-CDs for H₂O₂ generation catalyzed by uric acid, which was applied to the detection of uric acid in human urine. The sensing system had good linear range(5-100 μmol/L), low detection limit(1.8 μmol/L), satisfied recovery(96.8%-106.1%) and good relative standard deviation(less than 5%) in detection of uric acid. The results showed that Mo, S-CDs had advantages over natural peroxidases in terms of wide applicable temperature range and high stability, which would make them more widely useful in fields such as life analysis.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

制备MoS₂修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测

通讯作者: 牛娜,E-mail:niuna@nefu.edu.cn; 陈立钢,E-mail:ligangchen2008@163.com

English

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

Corresponding author: NIU Na, ; CHEN Li-gang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

制备MoS2修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测

通讯作者: 牛娜,E-mail:niuna@nefu.edu.cn; 陈立钢,E-mail:ligangchen2008@163.com

English

Preparation of MoS2-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

Corresponding author: NIU Na, ; CHEN Li-gang,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

制备MoS₂修饰的秸秆碳点纳米酶及对人尿液中尿酸含量的检测

Preparation of MoS₂-modified Straw Carbon Dots Nanozyme for Detection of Uric Acid Content in Human Urine

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs