基于蛋白质组学的鱼虾产品产地溯源分析

引用本文: 蒋冰雪, 张晓梅, 王志宏, 李兆杰, 赵雪, 徐杰, 侯虎, 赵飒, 张鸿伟, 薛长湖. 基于蛋白质组学的鱼虾产品产地溯源分析[J]. 分析化学, 2022, 50(4): 613-622. doi: 10.19756/j.issn.0253-3820.210834 shu

Citation: JIANG Bing-xue, ZHANG Xiao-mei, WANG Zhi-hong, LI Zhao-jie, ZHAO Xue, XU Jie, HOU Hu, ZHAO Sa, ZHANG Hong-wei, XUE Zhang-hu. Traceability Analysis of Geographical Origin of Fish and Shrimp Products Based on Proteomics[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 613-622. doi: 10.19756/j.issn.0253-3820.210834 shu

基于蛋白质组学的鱼虾产品产地溯源分析

蒋冰雪 ^1, ,
张晓梅 ^2, ,
王志宏 ^3, ,
李兆杰 ^1, ,
赵雪 ^1, ,
徐杰 ^1, ,
侯虎 ^1, ,
赵飒 ^2, ,
张鸿伟 ^{2,
,} ,
薛长湖 ^{1,4,
,}

1.
中国海洋大学食品科学与工程学院, 青岛 266003;2 青岛海关技术中心, 青岛 266114;3 青岛海洋食品营养与健康研究院, 青岛 266237;4 龙口海关综合技术服务中心, 龙口 265700

通讯作者: 张鸿伟,E-mail:zhanghongwei1@customs.gov.cn; 薛长湖,E-mail:xuech@ouc.edu.cn

基金项目:
青岛海关科研项目(Nos.QK201917,QK202101)资助。

国家重点研发计划重点专项项目(No.2018YFC0311206)

海关总署科技计划项目(No.2020HK209)

摘要: 基于相对定量的差异蛋白质组学技术，采用超高效液相色谱-四极杆/飞行时间质谱，比较了不同产地的南美白对虾、斑节对虾、鳕鱼和大西洋鲑鱼的蛋白质组。结合化学计量学工具，挖掘不同地理来源样本的蛋白质定量数据，以揭示蛋白质组差异。通过变量投射重要度、S-plot分析、刀切置信区间、差异倍数等参数筛选分析，得到南美白对虾、斑节对虾、鳕鱼和大西洋鲑鱼潜在的产地蛋白生物标志物各15、3、2和19种，可用于鉴别鱼虾产品的地理来源。本方法为重要水产品产地溯源提供了有效的技术工具，也为重要水产品进口贸易原产地监管和质量管控提供了基础数据。

关键词:

English

Traceability Analysis of Geographical Origin of Fish and Shrimp Products Based on Proteomics

1.
College of Food Science and Technology, Ocean University of China, Qingdao 266003, China;2 Technology Center of Qingdao Customs District, Qingdao 266114, China;3 Qingdao Institute for Nutrition and Health Innovation, Qingdao 266237, China;4 Longkou Customs Integrated Technical Service Center, Longkou 265700, China

Corresponding author: ZHANG Hong-wei, ; XUE Zhang-hu,

Received Date: 11 November 2021
Revised Date: 18 January 2022
Fund Project:

Supported by the Scientific Program of the General Administration of Customs of China（ No. 2020HK209), the National Key Research and Development Program of China（ No. 2018YFC0311206） and the Research Project of Qingdao Customs District（ Nos. QK201917, QK202101).

Abstract: The proteomes of white shrimp(Penaeus vannamei), black tiger shrimp(Penaeus monodon), cod(Dissostichus eleginoides) and Atlantic salmon(Salmo salar)from different geographical origins were investigated using ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry based on relatively quantitative differential proteomics. The quantitative protein data of samples from different geographical origins were mined by chemometric tools to reveal proteomic differences. By screening analysis of parameters such as variable importance in projection, S-plot analysis, knife-cut confidence interval and fold change, 15, 3, 2 and 19 potential protein biomarkers corresponding to Penaeus vannamei, Penaeus monodon, Dissostichus eleginoides and Salmo salar were obtained respectively for identifying the geographical origin of fish and shrimp products. The proposed protocol provided an alternative method for geographical provenance of important aquaculture products in international trade and basic data for supporting official control of geographical origin as well as quality controls of aquaculture products.

Key words:

Shrimp
/ Cod
/ Salmon
/ Proteomics
/ Geographical origin

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于蛋白质组学的鱼虾产品产地溯源分析

通讯作者: 张鸿伟,E-mail:zhanghongwei1@customs.gov.cn; 薛长湖,E-mail:xuech@ouc.edu.cn

English

Traceability Analysis of Geographical Origin of Fish and Shrimp Products Based on Proteomics

Corresponding author: ZHANG Hong-wei, ; XUE Zhang-hu,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于蛋白质组学的鱼虾产品产地溯源分析

通讯作者: 张鸿伟,E-mail:zhanghongwei1@customs.gov.cn; 薛长湖,E-mail:xuech@ouc.edu.cn

English

Traceability Analysis of Geographical Origin of Fish and Shrimp Products Based on Proteomics

Corresponding author: ZHANG Hong-wei, ; XUE Zhang-hu,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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