靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

引用本文: 陈雨湉, 任一平, 王丽丽, 黄忠平. 靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究[J]. 分析化学, 2021, 49(9): 1523-1530. doi: 10.19756/j.issn.0253-3820.201768 shu

Citation: CHEN Yu-Tian, REN Yi-Ping, WANG Li-Li, HUANG Zhong-Ping. Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1523-1530. doi: 10.19756/j.issn.0253-3820.201768 shu

靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

陈雨湉 ^1, ,
任一平 ^2, ,
王丽丽 ^{1,
,} ,
黄忠平 ^{1,
,}

1.
浙江工业大学化学工程学院, 杭州 310014;
2.
清华长三角研究院分析测试中心, 嘉兴 314006

通讯作者: 王丽丽,E-mail:lili_wang@zjut.edu.cn; 黄忠平,E-mail:zphuang@zjut.edu.cn

摘要: 基于靶向蛋白质组学技术，采用超高效液相色谱-串联高分辨质谱/串联三重四极杆质谱，建立了一种鉴别奶牛、水牛、牦牛、山羊、绵羊、马、驴和骆驼共8个物种奶的分析方法。针对8个物种奶中6种乳蛋白酶解产生的特征多肽进行分析，利用同位素内标法实现多条多肽的同时检测及比较，通过理论多肽分析、实际多肽检测、干扰考察、线性与最大酶解时间比较，最终筛选出13条特征多肽。依据13条特征多肽对模拟混合奶样品中的不同物种奶进行分析，对市售16个奶制品中的奶源物种进行鉴别。结果表明，特征多肽在奶源物种鉴别中具有良好的稳定性与特异性，本方法对多物种奶制品行业的质量控制与监管具有参考价值。

关键词:

English

Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products

1.
College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2.
Yangtze Delta Region Institute of Tsinghua University, Zhejiang Analysis and Test Center, Jiaxing 314006, China

Corresponding author: WANG Li-Li, ; HUANG Zhong-Ping,

Received Date: 18 December 2020
Revised Date: 22 April 2021

Abstract: A method based on target proteomics and ultrahigh performance liquid chromatography-high resolution mass spectrometry/tandem mass spectrometry was established for species identification of milks and dairies from cow, buffalo, yak, goat, sheep, horse, donkey and camel. Tryptic hydrolyzed peptides from 6 kinds of milk proteins in 8 species were analyzed and screened through theoretical analysis, instrument examination, investigation of interferences, linearity and digestion speed. A total of 13 specific peptides were finally chosen and applied to identification and detection of multi-species for milk mixture samples and commercial dairies. The results showed that specific peptides maintained good stability and specificity during species analysis. This method provided valuable suggestion in quality control and supervision of multi-species diary industry.

Key words:

Targeted proteomics
/ High performance liquid chromatography-mass spectrometry
/ Species identification
/ Milk protein

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

通讯作者: 王丽丽,E-mail:lili_wang@zjut.edu.cn; 黄忠平,E-mail:zphuang@zjut.edu.cn

English

Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products

Corresponding author: WANG Li-Li, ; HUANG Zhong-Ping,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

靶向蛋白质组学技术在多物种奶及其奶制品鉴别中的应用研究

通讯作者: 王丽丽,E-mail:lili_wang@zjut.edu.cn; 黄忠平,E-mail:zphuang@zjut.edu.cn

English

Application of Targeted Proteomics in Species Identification of Milk Proteins in Multi-species Milk Products

Corresponding author: WANG Li-Li, ; HUANG Zhong-Ping,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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