聚丙烯酸-Tb@SiO<sub>2</sub>聚合物纳米粒子用于炭疽生物标志物检测

引用本文: 邵康, 凌青青, 施姝娴, 叶十一, 滕渊洁, 潘再法. 聚丙烯酸-Tb@SiO₂聚合物纳米粒子用于炭疽生物标志物检测[J]. 分析化学, 2021, 49(5): 726-732. doi: 10.19756/j.issn.0253-3820.201381 shu

Citation: SHAO Kang, LING Qing-Qing, SHI Shu-Xian, YE Shi-Yi, TENG Yuan-Jie, PAN Zai-Fa. Synthesis of Polyacrylic-Tb@SiO₂ Polymer Nanoparticles for Detection of Anthrax Biomarkers[J]. Chinese Journal of Analytical Chemistry, 2021, 49(5): 726-732. doi: 10.19756/j.issn.0253-3820.201381 shu

聚丙烯酸-Tb@SiO₂聚合物纳米粒子用于炭疽生物标志物检测

邵康 ^1, ,
凌青青 ^1, ,
施姝娴 ^1, ,
叶十一 ^2, ,
滕渊洁 ^1, ,
潘再法 ^{1,
,}

1.
浙江工业大学化学工程学院, 杭州 310014;
2.
长江大学生命科学学院, 荆州 434000

通讯作者: 潘再法,E-mail:panzaifa@zjut.edu.cn

基金项目:
国家自然科学基金项目（No.21804119）、浙江省自然科学基金项目（No.LZ18B050002）和湖北省科技计划项目（No.2018CFB388）资助。

摘要: 构建了一种对炭疽生物标志物2，6-吡啶二羧酸（DPA）具有高选择性的聚丙烯酸-Tb@SiO₂（PAA-Tb@SiO₂）荧光纳米探针。探针中的Tb³⁺能与DPA形成三配位结构，进而产生"天线效应"，使探针荧光增强，可以实现对DPA的高灵敏检测。在0.1~20.0 μmol/L浓度范围内，探针的荧光强度与DPA浓度呈良好的线性关系，相关系数（R²）为0.9978，检出限（3σ）为6 nmol/L。采用本方法实现了自来水中DPA的测定，加标回收率在94.6%~116.8%之间，RSD<2%。本方法灵敏度高、选择性好，对环境水样中炭疽芽孢杆菌的检测具有潜在的应用价值。

关键词:

English

Synthesis of Polyacrylic-Tb@SiO₂ Polymer Nanoparticles for Detection of Anthrax Biomarkers

1.
School of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
2.
School of Life Science, Yangtze University, Jingzhou 434000, China

Corresponding author: PAN Zai-Fa, panzaifa@zjut.edu.cn

Received Date: 01 July 2020
Revised Date: 01 February 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No. 21804119), the Natural Science Foundation of Zhejiang Province, China (No. LZ18B050002), and the Science and Technology Plan Project of Hubei Province, China (No. 2018CFB388).

Abstract: A novel poly(acrylic acid)-Tb@SiO₂ (PAA-Tb@SiO₂) fluorescent probe was constructed for detection of 2,6-pyridinedicarboxylic acid (DPA) that was regarded as the potential biomarkers of anthrax. The DPA could coordinate with Tb³⁺ in this probe, which produced an "antenna effect" to enhance the fluorescence intensity of the probe. There was a good linear relationship between fluorescence intensity and DPA concentration in the range of 0.1-20.0 μmol/L, and the correlation coefficient (R²) was 0.9978. The detection limit was 6 nmol/L (3σ). The established method realized the detection of DPA in tap water, the recoveries of the spike were between 94.6% and 116.8%, and the RSD was less than 2%. The method had high sensitivity and good selectivity, and could be used for the analysis of anthrax in environmental water samples.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

聚丙烯酸-Tb@SiO₂聚合物纳米粒子用于炭疽生物标志物检测

通讯作者: 潘再法,E-mail:panzaifa@zjut.edu.cn

English

Synthesis of Polyacrylic-Tb@SiO₂ Polymer Nanoparticles for Detection of Anthrax Biomarkers

Corresponding author: PAN Zai-Fa, panzaifa@zjut.edu.cn

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通讯作者: 陈斌, bchen63@163.com

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聚丙烯酸-Tb@SiO2聚合物纳米粒子用于炭疽生物标志物检测

通讯作者: 潘再法,E-mail:panzaifa@zjut.edu.cn

English

Synthesis of Polyacrylic-Tb@SiO2 Polymer Nanoparticles for Detection of Anthrax Biomarkers

Corresponding author: PAN Zai-Fa, panzaifa@zjut.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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聚丙烯酸-Tb@SiO₂聚合物纳米粒子用于炭疽生物标志物检测

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