一种超灵敏检测镉离子的核酸适配体电化学传感器

引用本文: 袁敏, 钱世权, 曹慧, 徐斐, 叶泰, 于劲松, 郭文, 吴嘉颖, 阿提坎·吾斯曼. 一种超灵敏检测镉离子的核酸适配体电化学传感器[J]. 分析化学, 2020, 48(12): 1701-1708. doi: 10.19756/j.issn.0253-3820.201072 shu

Citation: YUAN Min, QIAN Shi-Quan, CAO Hui, XU Fei, YE Tai, YU Jin-Song, GUO Wen, WU Jia-Ying, A Ti-kan·Wu-siman. An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺[J]. Chinese Journal of Analytical Chemistry, 2020, 48(12): 1701-1708. doi: 10.19756/j.issn.0253-3820.201072 shu

一种超灵敏检测镉离子的核酸适配体电化学传感器

袁敏 ^1, ,
钱世权 ^1, ,
曹慧 ^1, ,
徐斐 ^{1,2,
,} ,
叶泰 ^1,2, ,
于劲松 ^1,2, ,
郭文 ^1, ,
吴嘉颖 ^1, ,
阿提坎·吾斯曼 ^1,

1.
上海理工大学医疗器械与食品学院, 上海食品快速检测工程技术研究中心, 上海 200093;
2.
上海理工大学食品质量与安全研究所, 上海 200093

通讯作者: 徐斐, xufei8135@126.com

基金项目:
本文系国家自然科学基金项目（Nos.31671934，61501295）、科技部"十三五"计划项目（No.2017YFC1600603）和上海市科委重点攻关项目（Nos.18391901200，17391901500）资助

摘要: 设计了一种基于核酸适配体检测镉离子（Cd²⁺）的电化学生物传感器，将适配体互补链（CDNA）通过AuS键自组装于金电极表面，并与适配体杂交结合形成双链DNA。由于适配体对Cd²⁺有特异性结合能力，加入Cd²⁺后，与互补链竞争结合适配体，使修饰二茂铁基团的适配体从金电极表面脱落，二茂铁的电化学信号显著减小。采用方波伏安法（SWV）进行检测，本传感器对Cd²⁺的线性检测范围为1.0 nmol/L~10.0 μmol/L，检出限为65.1 pmol/L，线性方程为ΔI=0.2872+0.2327lgC（R²=0.9972），10 s内即可完成检测。实际江水样品中Cd²⁺的检测结果与石墨炉原子吸收光谱法的检测结果一致，加标回收率为97.1%~99.5%。本方法灵敏度高、检测速度快、特异性强，在镉环境污染监测方面具有良好的应用前景。

关键词:

English

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺

1.
School of Medical Instruments and Food Engineering, Shanghai Engineering Research Center for Food Rapid Detection, University of Shanghai for Science and Technology, Shanghai 200093, China;
2.
Institute of Food Quality and Safety, University of Shanghai for Science and Technology, Shanghai 200093, China

Corresponding author: XU Fei, xufei8135@126.com

Received Date: 13 February 2020
Revised Date: 14 August 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 31671934, 61501295), the Ministry of Science and Technology of China (No. 2017YFC1600603) and the Shanghai Committee of Science and Technology (Nos.18391901200, 17391901500).

Abstract: An aptamer-based electrochemical biosensor was developed for detection of cadmium ions (Cd²⁺). Firstly, the mercapto-modified complementary sequence (CDNA) of aptamer was immobilized on the electrode surface via AuS bond. Then, it hybridized with aptamer to form a double-stranded DNA. Due to the specific binding of aptamer and Cd²⁺, the aptamer labeled with ferrocene was competed off the gold electrode, so that the electrochemical signal of ferrocene decreased significantly. By using square wave voltammetry (SWV) detection and under the optimal conditions, a linear relationship was found between the current signal and logarithm of Cd²⁺ concentration with an equation of ΔI=0.2827+0.2372lgC (R²=0.9972), where the linearity range was from 1.0 nmol/L to 10.0 μmol/L, with a detection limit of 65.1 pmol/L, and the recoveries in real river samples were 97.1%-97.9%, which was comparable to those measured by graphite furnace atomic absorption spectrometry. The proposed method displayed many advantages such as high sensitivity, fast detection and excellent specificity, and had a good application prospect in monitoring cadmium pollution.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

一种超灵敏检测镉离子的核酸适配体电化学传感器

通讯作者: 徐斐, xufei8135@126.com

English

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺

Corresponding author: XU Fei, xufei8135@126.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一种超灵敏检测镉离子的核酸适配体电化学传感器

通讯作者: 徐斐, xufei8135@126.com

English

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd2+

Corresponding author: XU Fei, xufei8135@126.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd²⁺

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