基于核磁共振技术的临床尿毒症的代谢组学研究

引用本文: 王振召, 魏斌斌, 陈峥, 诸宏伟, 沈桂平, 冯江华. 基于核磁共振技术的临床尿毒症的代谢组学研究[J]. 分析化学, 2018, 46(9): 1415-1423. doi: 10.11895/j.issn.0253-3820.181286 shu

Citation: WANG Zhen-Zhao, WEI Bin-Bin, CHEN Zheng, ZHU Hong-Wei, SHEN Gui-Ping, FENG Jiang-Hua. ¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1415-1423. doi: 10.11895/j.issn.0253-3820.181286 shu

基于核磁共振技术的临床尿毒症的代谢组学研究

王振召 ^1, ,
魏斌斌 ^1, ,
陈峥 ^2, ,
诸宏伟 ^2, ,
沈桂平 ^1, ,
冯江华 ^1,

1.
厦门大学电子科学系, 福建省等离子体与磁共振重点实验室, 厦门 361005;
2.
蚌埠医学院第一附属医院, 蚌埠 233004
基金项目:
本文系国家自然科学基金项目（Nos.31671920，81272581）资助

摘要: 采用核磁共振技术与统计学分析方法，结合尿液的生化指标，进行临床尿毒症尿样的代谢组学研究。结果表明，尿毒症患者和健康对照人群尿样的代谢轮廓存在明显差异，通过核磁共振氢谱鉴定出70种代谢物，其中20种具有显著差异。与健康人群相比，尿毒症患者尿液中2-羟基异丁酸、3-羟基丁酸、丙酮、丁酸、谷氨酸、肌氨酸、肌酐、赖氨酸、N，N-二甲基组氨酸、柠檬酸、天冬酰胺、乙醇和乙醇胺的含量明显偏低，而支链氨基酸（包括亮氨酸、缬氨酸、异亮氨酸）、牛磺酸、乳酸、α-葡萄糖和β-葡萄糖的含量明显偏高。这些代谢物涉及氨基酸代谢、能量代谢和脂质代谢中的多条代谢途径，可作为尿毒症代谢影响的潜在的生物标志物，有助于理解尿毒症发病的生化机制。

关键词:

English

¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis

1.
Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China;
2.
The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China
Received Date: 30 April 2018
Revised Date: 15 July 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 31671920, 81272581).

Abstract: At present, little is known about metabolic mechanism of uremia. In this study, uremia was investigated by combination of NMR spectroscopy, statistical analysis and urinary biochemical assays to provide the basis of the metabolic characteristics of uremia. The characteristic metabolites of uremia were identified from the urine of the uremia patients. The results showed that the urinary levels of 2-hydroxyisobutyrate, 3-hydroxybutyrate, acetone, butyrate, glutamate, sarcosine, creatinine, lysine, N,N-dimethylglycine, citrate, asparagine, ethanol and ethanolamine were reduced, while the urinary levels of branched-chain amino acids (including leucine, valine and isoleucine), taurine, lactate and glucose were higher in uremia patients than that in the healthy subjects. The corresponding disturbed metabolic pathways were derived from the characteristic metabolites, in which amino acid, energy and lipid metabolisms were involved. The specific characteristic metabolites will help to understand the underlying biochemical mechanisms of the onset of uremia.

Key words:

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基于核磁共振技术的临床尿毒症的代谢组学研究

English

¹H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis

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基于核磁共振技术的临床尿毒症的代谢组学研究

English

1H Nuclear Magnetic Resonance-based Investigation of Uremia by Metabolomic Analysis

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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