液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

引用本文: 李慧, 鲁文慧, 焦丽丽, 吴巍, 宋凤瑞, 刘淑莹. 液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量[J]. 分析化学, 2018, 46(4): 586-593. doi: 10.11895/j.issn.0253-3820.171472 shu

Citation: LI Hui, LU Wen-Hui, JIAO Li-Li, WU Wei, SONG Feng-Rui, LIU Shu-Ying. Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 586-593. doi: 10.11895/j.issn.0253-3820.171472 shu

液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

1.
中国科学院长春应用化学研究所, 长春 130022;
2.
长春中医药大学吉林省人参科学研究院, 长春 130117
基金项目:
本文系吉林省科技发展计划基金项目（Nos.20160308YY，20160101334JC）和吉林省自然科学基金项目（No.20180520062JH）资助

摘要: 建立了一种液相色谱-质谱联用技术同时检测γ-氨基丁酸（GABA）、5-羟色胺（5-HT）、酪氨酸（L-tyr）、多巴胺（DA）、去甲肾上腺素（NE）和肾上腺素（E）6种神经递质的方法，并将本方法用于大鼠肾上腺、下丘脑及血浆中神经递质的检测。以丹磺酰氯为衍生化试剂，以5-羟基色氨酸和咖啡酸为内标，采用UltiMate 3000 RSLC色谱系统、Thermo C₁₈色谱柱（50 mm×3 mm，2.7 μm），流动相为0.1%甲酸和甲醇溶液梯度洗脱，流速0.2 mL/min，进样量2 μL，多反应检测（MRM），正离子模式。GABA、5-HT、L-Tyr、DA、NE、E线性检测范围分别为0.26~620.80 μmol/L、0.034~11.20 μmol/L、1.20~88.00 μmol/L、0.03~41.02 μmol/L、0.01~47.20 μmol/L、0.01~90.24 μmol/L，加标回收率为91.16%~116.20%。本方法可快速准确检测大鼠肾上腺、血浆及下丘脑中神经递质含量，为药理实验的中的指标分析提供了检测技术支持。

关键词:

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

1.
Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China;
2.
Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China
Received Date: 02 December 2017
Revised Date: 05 January 2018

Abstract: A rapid Liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of six neurotransmitters in rat plasma, adrenal gland and hypothalamus, including γ-aminobutyric acid (GABA), serotonin(5-HT), tyrosine(L-Tyr), dopamine(DA), norepinephrine(NE) and epinephrine(E). This method was used for the neurotransmitters detection in rat adrenal, blood and hypothalamus. The samples were pre-column derivatized with dansyl chloride, and 5-HTP and CA were used as internal standards. A Thermo C₁₈ column (50 mm×3 mm, 2.7 μm) was used for sample separation with 0.1% aqueous formic acid solution as phase A and methanol as phase B at a flow rate of 0.2 mL/min, and the injection volume was set at 2 μL. The detection was carried out with multi-reaction monitoring (MRM) in electron spray ionization (ESI) positive mode. The linear ranges for detection of GABA, 5-HT, L-Tyr, DA, NE and E were 0.26-620.80 μmol/L, 0.03-11.20 μmol/L, 1.20-88.00 μmol/L, 0.03-41.02 μmol/L, 0.01-47.20 μmol/L, 0.01-90.24 μmol/L, respectively, with recoveries varying from 91.16% to 116.20%. This method can detect the neurotransmitters rapidly and accurately, providing a platform for the determination of neurotransmitters in rat plasma, adrenal gland and hypothalamus in pharmacological experiments.

Key words:

Liquid chromatography-tandem mass spectrometry
/ Adrenal
/ Blood
/ Hypothalamus
/ Neurotransmitters

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液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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