直线式基质辅助激光解吸电离质谱仪的研制与性能表征

引用本文: 喻佳俊, 刘平, 曾真, 陈颖, 高伟, 李梅, 王晨光, 黄正旭, 周振, 李磊. 直线式基质辅助激光解吸电离质谱仪的研制与性能表征[J]. 分析化学, 2018, 46(4): 463-470. doi: 10.11895/j.issn.0253-3820.171415 shu

Citation: YU Jia-Jun, LIU Ping, ZENG Zhen, CHEN Ying, GAO Wei, LI Mei, WANG Chen-Guang, HUANG Zheng-Xu, ZHOU Zhen, LI Lei. Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 463-470. doi: 10.11895/j.issn.0253-3820.171415 shu

直线式基质辅助激光解吸电离质谱仪的研制与性能表征

喻佳俊 ^1, ,
刘平 ^3, ,
曾真 ^1, ,
陈颖 ^1, ,
高伟 ^1,2, ,
李梅 ^1,2, ,
王晨光 ^4, ,
黄正旭 ^1,2, ,
周振 ^1,2, ,
李磊 ^1,2,

1.
暨南大学质谱仪器与大气环境研究所, 广州 510632;
2.
广东省大气污染在线源解析系统工程技术研究中心, 广州 510632;
3.
广州禾信仪器股份有限公司, 广州 510530;
4.
中国科学院广州能源研究所, 广州 510640
基金项目:
本文系广东省应用型科技研发专项资金（No.2015B020236003）、中央高校基本科研业务费专项资金（No.21616341）资助

摘要: 基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）是一种能够对蛋白质、多肽、核酸等生物大分子进行定性分析的质谱仪器，近年来在微生物快速鉴定领域引起了广泛关注。本研究结合应用需求研制了一台直线式MALDI-TOF MS仪器。仪器主要由真空系统、快速进样系统、光学系统、离子源、飞行时间质量分析器、数据采集系统及电控系统组成。离子源采用双场加速、延迟引出及动态脉冲聚焦技术，整个离子的无场飞行距离为1 m。光学系统采用长寿命的固体激光器，可实现频率1~2000 Hz、聚焦光斑20~100 μm连续可调，能量0~80 μJ连续可调。激光引入光路与离子飞行轴线的角度控制在5°内，有效提高了仪器的灵敏度和分辨率。对仪器进行性能测试表明，可检测的质量数超过199 kDa，在1000~3000 Da范围内可达到同位素分辨，在5000~17000 Da范围内分辨率可达900（FWHM），实现了宽质量范围内的良好分辨率。灵敏度测试表明，仪器对短杆菌肽的最低检测浓度为10 amol/μL，绝对灵敏度可达2.56 amol。不同靶点的唾液样品独立检测结果表明，仪器具有较高的结果重现性。此外，对两种遗传特性相似的大肠埃希氏菌和志贺菌进行检测的结果表明，此仪器能够对这两种特征相似的细菌成功检测和鉴定，具有临床微生物鉴定的潜力。

关键词:

English

Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer

1.
Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, China;
2.
Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, Guangzhou 510632, China;
3.
Guangzhou HeXin Instrument Co., LTD, Guangzhou 510530, China;
4.
Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China
Received Date: 11 November 2017
Revised Date: 01 December 2017

Abstract: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an important analytical technique for biological macromolecules, such as proteins, peptides and nucleic acid, especially in the field of microbial identification. Based on previous study, a linear MALDI-TOF MS has been designed and assembled for biological applications. The instrument comprises a vacuum system, a vacuum fast sample introduction system, an optical system, a time-of-flight mass analyzer, an ion source, a data acquisition system and an electric control system. The ion source adopts two-stage source acceleration, delayed extraction and dynamic pulse focusing technology. The time-of-flight distance of field-free drift region is about 1 meter. The optical system adopts a solid laser with adjustable frequency of 1-2000 Hz and spots of 20-100 μm. The angle of incidence laser is controlled at 5 degrees. A series of experiments are carried out to further evaluate the instrument performances. It can not only analyze the samples more than 199 kDa, but also achieve isotope resolution at 1000-3000 Da and up to 900(FWHM) at 5000-17000 Da. The minimum detectable concentration of gramicidin is 10 amol/μL, absolute sensitivity can reach up to 2.56 amol. Independent detection of saliva samples from different targets shows that the instrument has high reproducibility. We identified Escherichia Coli and Shigella spp, which are two common bacteria but difficult to be differentiated by mass spectrometry, showing its potential identification for clinical microorganism. In a summary, this instrument can play a role on clinical examination in the near future.

Key words:

Matrix-assisted laser desorption ionization
/ Linear time-of-flight mass spectrometer
/ Development
/ Performance characterization

1. [1]
  Fenn J B, Mann M, Meng C K, Wong S F, Whitehouse C M. Science,1989,246(4926):64-71
2. [2]
  Karas M, Hillenkamp F. Anal. Chem.,1988,60(20):2299-2301
3. [3]
  Zhu X Q, Huang Z X, Gao W, Li X, Li L, Zhu H, Mo T, Huang B, Zhou Z. J. Agric. Food Chem.,2016,64(27):5614-5619
4. [4]
  WANG Zhong-Hua, CHEN Yan-Hua, XU Jing, CHEN Hui-Li, ZHOU Xia, XIAO Xin-Hua, PING Fan, HE Jiu-Ming, ABLIZ Zeper, ZHANG Rui-Ping. Chinese J. Anal.Chem., 2017,45(5):654-661 王中华, 陈艳华, 徐婧, 陈蕙莉, 周霞, 肖新华, 平凡, 贺玖明, 再帕尔·阿不力孜, 张瑞萍.分析化学,2017,45(5):654-661
5. [5]
  Vestal M L. J. Am. Soc. Mass Spectrom.,2011,22(6):953-959
6. [6]
  Prentice B M, Chumbley C W, Hachey B C, Norris J L, Caprioli R M. Anal. Chem.,2016,88(19):9780-9788
7. [7]
  Medzihradszky K F, Campbell J M, Baldwin M A, Falick A M, Juhasz P, Vestal M L, Burlingame A L. Anal. Chem.,2000,72(3):552-558
8. [8]
  Vestal M L, Hayden K. Int. J. Mass Spectrom.,2007,268(2):83-92
9. [9]
  Karas M, Kruger R. Chem. Rev.,2003,103(2):427-440
10. [10]
  Spengler B, Kirsch D, Kaufmann R. J. Phys. Chem.,1992,96(24):9678-9684
11. [11]
  Tran B Q, Barton C, Feng J H, Sandjong A, Yoon S H, Awasthi S, Liang T, Khan M M, Kilgour D P A, Goodlett D R, Goo Y A. J. Proteomics,2016,134:93-101
12. [12]
  Rodrigo M A M, Zitka O, Krizkova S, Moulick A, Adam V, Kizek R. J. Pharm. Biomed. Anal.,2014,95:245-255
13. [13]
  Meyer S, Vollmert C, Trost N, Sigurdardottir S, Portmann C, Gottschalk J, Ries J, Markovic A, Infanti L, Buser A. Br. J. Haematol.,2016,174(4):624-636
14. [14]
  Fenselau C, Demirev P A. Mass Spectrom. Rev.,2001,20(4):157-171
15. [15]
  Saffert R T, Cunningham S A, Ihde S M, Jobe K E M, Mandrekar J, Patel R. J. Clin. Microbiol.,2011,49(3):887-892
16. [16]
  Davies A P, Reid M, Hadfield S J, Johnston S, Mikhail J, Harris L G, Jenkinson H F, Berry N, Lewis A M, El-Bouri K, Mack D. J. Clin. Microbiol.,2012,50(12):4087-4090
17. [17]
  Mather C A, Rivera S F, Butler-Wu S M. J. Clin. Microbiol.,2014,52(1):130-138
18. [18]
  Ferrand J, Hochard H, Girard V, Aissa N, Bogard B, Alauzet C, Lozniewski A. J. Clin. Microbiol.,2016,54(3):782-784
19. [19]
  Vestal M L. Clin. Chem.,2016,62(1):20-23
20. [20]
  Hattan S J, Parker K C, Vestal M L, Yang J Y, Herold D A, Duncan M W. J. Am. Soc. Mass Spectrom.,2016,27(3):532-541
21. [21]
  Brown R S, Lennon J J. Anal. Chem.,1995,67(13):1998-2003
22. [22]
  Franzen J. Int. J. Mass Spectrom. Ion. Processes.,1997,164(1):19-34
23. [23]
  Bökelmann V, Spengler B, Kaufmann R. Eur. J. Mass Spectrom.,1995,1(1):81-93
24. [24]
  Aksouh F, Chaurand P, Deprun C, Della-Negra S, Beyec Y L, Hoyes J, Pinho R R. Rapid Commun. Mass Spectrom.,1995,9(6):515-518
25. [25]
  Keys C J, Dare D J, Sutton H, Wells G, Lunt M, McKenna T, McDowall M, Shah H N. Infect. Genet. Evol.,2004,4(3):221-242
26. [26]
  Sauer S, Kliem M. Nat. Rev. Microbiol.,2010,8(1):74-82
27. [27]
  Arnold R J, Reilly J P. Anal. Biochem.,1999,269(1):105-112
28. [28]
  Khot P D, Fisher M A. J. Clin. Microbiol.,2013,51(11):3711-3716

扫一扫看文章

计量

PDF下载量: 18
文章访问数: 1422
HTML全文浏览量: 261

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

直线式基质辅助激光解吸电离质谱仪的研制与性能表征

English

Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

直线式基质辅助激光解吸电离质谱仪的研制与性能表征

English

Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs