一种亲水性温敏聚合物荧光探针的制备及用于农产品中铝离子的检测

引用本文: 解雪乔, 蔡云凤, 韩娟, 王赟, 陈桐, 李静. 一种亲水性温敏聚合物荧光探针的制备及用于农产品中铝离子的检测[J]. 分析化学, 2018, 46(4): 502-510. doi: 10.11895/j.issn.0253-3820.171392 shu

Citation: XIE Xue-Qiao, CAI Yun-Feng, HAN Juan, WANG Yun, CHEN Tong, LI Jing. Synthesis of A New Type of Responsive Hydrophilic Block Copolymer Fluorescence Probe and Its Application in Detection of Aluminum Ion in Agricultural Products[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 502-510. doi: 10.11895/j.issn.0253-3820.171392 shu

一种亲水性温敏聚合物荧光探针的制备及用于农产品中铝离子的检测

解雪乔 ^1, ,
蔡云凤 ^2, ,
韩娟 ^2, ,
王赟 ^3, ,
陈桐 ^4, ,
李静 ^4,

1.
江苏省苏州健雄职业技术学院医药科技学院, 太仓 215400;
2.
江苏大学食品科学与工程学院, 镇江 212013;
3.
江苏大学化学化工学院, 镇江 212013;
4.
镇江出入境检验检疫局, 镇江 212008
基金项目:
本文系太仓市科技发展计划项目（No.TC2015NY05）、江苏省高校自然科学研究面上项目（16KJB550010）、镇江市重点研发计划项目（No.SH2016019）和江苏出入境检验检疫局科技计划项目（No.2017KJ47）资助

摘要: 设计合成了一种亲水性温敏聚合物荧光探针，用于检测农产品中的Al³⁺。通过可逆加成-断裂链转移（RAFT）聚合合成此聚合物荧光探针（L64-BTPA-SHMA），用¹H NMR对其进行表征，并通过荧光光谱对聚合物探针的识别性能进行了研究。结果表明，此聚合物荧光探针检测Al³⁺的荧光响应信号受温度影响显著，温度越高，荧光强度越低。聚合物荧光探针在25℃和pH 7.4的缓冲溶液中对Al³⁺有良好的选择性识别作用，且基本不受其它金属离子的影响。建立了基于此聚合物探针对Al³⁺的荧光检测方法，响应信号与Al³⁺浓度在2.0~18.0 μmol/L范围内呈线性关系，相关系数为0.9977，检出限为1.43 μmol/L。此聚合物荧光探针可应用于检测农产品中Al³⁺残留，具有良好的实际应用价值。

关键词:

English

Synthesis of A New Type of Responsive Hydrophilic Block Copolymer Fluorescence Probe and Its Application in Detection of Aluminum Ion in Agricultural Products

1.
Department of Medical Science and Technology, Suzhou Chien-shiung Institute of Technology, Taicang 215400, China;
2.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
3.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China;
4.
Zhenjiang Entry-Exit Inspection and Quarantine Bureau, Zhenjiang 212008, China
Received Date: 06 November 2017
Revised Date: 19 January 2018
Fund Project:
This work was supported by the Project of Science and Technology Development Plan of Taicang (No.TC2015NY05), the Natural Research Project of the Jiangsu Higher Education Institutions of China (No.16KJB550010), Zhenjiang Social Development Project (No.SH2016019) and the Science Foundation of Jiangsu Entry-exit Inspection Quarantine Bureau (No.2017KJ47).

Abstract: A temperature-responsive hydrophilic block copolymer fluorescence sensor based on selective analysis of Al³⁺ was designed and synthesized. The polymer fluorescent probe (L64-BTPA-SHMA) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization and characterized by ¹H NMR, and the recognition performance of the polymer probe was studied by fluorescence spectroscopy. It was found that the fluorescence response of Al³⁺ detected by this polymer fluorescence probe was significantly affected by temperature. The higher the temperature, the lower the fluorescence intensity was observed. The results showed that 0.1 g/L polymer fluorescent probe had a good selectivity to Al³⁺ in buffer solution at 25℃ and pH 7.4, and was not affected by other metal ions. A fluorescence detection method based on this polymer probe for Al³⁺ was developed. In this method, the fluorescence intensity was linear with Al³⁺ concentration in the range of 2.0-18.0 μmol/L, with a correlation coefficient of 0.9977 and a detection limit of 1.43 μmol/L. The fluorescence response between the polymer fluorescence chemosensor and Al³⁺ could be altered by controlling the temperature change. The successful application of the polymer fluorescence probe for detecting Al³⁺ residues in agricultural products has practical application value.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

一种亲水性温敏聚合物荧光探针的制备及用于农产品中铝离子的检测

English

Synthesis of A New Type of Responsive Hydrophilic Block Copolymer Fluorescence Probe and Its Application in Detection of Aluminum Ion in Agricultural Products

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一种亲水性温敏聚合物荧光探针的制备及用于农产品中铝离子的检测

English

Synthesis of A New Type of Responsive Hydrophilic Block Copolymer Fluorescence Probe and Its Application in Detection of Aluminum Ion in Agricultural Products

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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