表面电性可控磁珠微流控芯片在DNA提取中的应用

引用本文: 黄华斌, 傅奇, 胡佳, 游其华, 庄峙厦, 杨朝勇, 王小如. 表面电性可控磁珠微流控芯片在DNA提取中的应用[J]. 分析化学, 2018, 46(9): 1372-1378. doi: 10.11895/j.issn.0253-3820.171318 shu

Citation: HUANG Hua-Bin, FU Qi, HU Jia, YOU Qi-Hua, ZHUANG Zhi-Xia, YANG Chao-Yong, WANG Xiao-Ru. Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1372-1378. doi: 10.11895/j.issn.0253-3820.171318 shu

表面电性可控磁珠微流控芯片在DNA提取中的应用

1.
厦门华厦学院检验与科学技术系, 厦门 361024;
2.
厦门大学化学化工学院, 厦门 361005
基金项目:
本文系国家星火计划项目（No.2015GA721002）、黄土与第四纪地质国家重点实验室开放基金（No.SKLLQG1607）和福建省中青年教师科技计划项目（No.JAT170817）资助

摘要: 制备了表面电性可控的氨基化SiO₂@Fe₃O₄磁性复合微球，采用激光刻蚀和热压键合的方法制作了聚甲基丙烯酸甲酯（PMMA）材质的DNA固相萃取芯片，将磁珠灌注于芯片通道中，借助永磁铁固定并控制磁珠，将磁珠芯片应用于人类全血中的基因组DNA提取，优化了提取实验条件，并对提取产物进行凝胶电泳和PCR分析。实验结果表明，磁珠微流控芯片成功地从全血中提取出纯度较高的基因组DNA，提取效率约35%，提取液的凝胶电泳条带与商品化试剂盒提取的基因组DNA一致，提取液可用于进一步的PCR反应。

关键词:

English

Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction

1.
XiaMen HuaXia College, Department of Science and Technology for Inspection, Xiamen 361024, China;
2.
College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Received Date: 10 October 2017
Revised Date: 08 July 2018
Fund Project:
This work was supported by the National Spark Program (No. 2015GA721002), the Open Foundation from State Key Laboratiory of Loess and Quaternary Geology(No. SKLLQG1607) and the Project of Science and Technology for Young Teachers in Fujian Province, China(No. JAT170817)

Abstract: Self-made surface charge switching amino-SiO₂@Fe₃O₄ magnetic composite microspheres were applied to microfluidic chip, and DNA solid phase extraction chip of polymethyl methacrylate (PMMA) was fabricated by laser etching and hot pressing bonding. The magnetic beads that could be controlled by permanent magnet were injected into the chip channel to obtain a magnetic bead chip for genomic DNA extraction in human whole blood. Under the optimized extraction conditions (with 80 μg beads, flow rate for adsorption, elution and desorption were 10, 5 and 20 μL/min, respectively), the extracted products were subjected to gel electrophoresis and PCR test. The results showed that the genomic DNA with high purity was extracted from the whole blood by magnetic bead microfluidic chip. The extraction efficiency was about 35%. The gel electrophoresis band of the extract was consistent with the genomic DNA extracted by commercial reagent kit. The extract solution could be used for further PCR reaction.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

表面电性可控磁珠微流控芯片在DNA提取中的应用

English

Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

表面电性可控磁珠微流控芯片在DNA提取中的应用

English

Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

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