脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

引用本文: 吕扬, 张雅文, 谭磊, 纪文亮, 于萍, 毛兰群, 周方. 脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析[J]. 分析化学, 2017, 45(11): 1595-1599. doi: 10.11895/j.issn.0253-3820.171091 shu

Citation: LYU Yang, ZHANG Ya-Wen, TAN Lei, JI Wen-Liang, YU Ping, MAO Lan-Qun, ZHOU Fang. Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1595-1599. doi: 10.11895/j.issn.0253-3820.171091 shu

脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

吕扬 ^1, ,
张雅文 ^1, ,
谭磊 ^1, ,
纪文亮 ^2, ,
于萍 ^2, ,
毛兰群 ^2, ,
周方 ^1,

1.
北京大学第三医院, 北京 100191;
2.
中国科学院化学研究所活体分析化学重点实验室, 北京 100190
基金项目:
本文系北京市科技计划项目（新）（No.2144000021）资助

摘要: 利用活体微透析技术结合在线高选择性抗坏血酸电化学检测技术，以实验性胸10节段脊髓钳夹损伤为动物模型，研究了脊髓损伤过程中胞外抗坏血酸的变化规律。结果表明，微透析探针植入及钳夹损伤瞬间，均存在脊髓抗坏血酸浓度高峰，随后浓度回到稳定水平，对照组大鼠脊髓细胞外液中抗坏血酸的平均基础浓度为（26.17±1.25）μmol/L （n=8）。实验组脊髓钳夹损伤后，胞外抗坏血酸水平显著增加，达到（53.24±1.95）μmol/L （n=8），这种显著性升高可能与继发性脊髓损伤保护机制导致胞内抗坏血酸外流相关。本研究结果为抗坏血酸参与调节继发性脊髓损伤提供了直接的实验证据。

关键词:

English

Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model

1.
Peking University Third Hospital, Beijing 100191, China;
2.
Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
Received Date: 13 July 2017
Revised Date: 13 September 2017
Fund Project:
This work was supported by the Beijing Science and Technology Project (New), China (No.2144000021).

Abstract: Acute traumatic spinal cord injury (SCI) represents one of the most devastating injuries that afflict the human body. Ascorbic acid (AA) plays an important role in mammalian central nervous system, especially in SCI. In this study, the change of AA concentration after SCI was investigated by using an on-line electrochemical method integrated with in vivo microdialysis. A microdialysis probe (2 mm in length) was implanted into the spinal cord of an anesthetized rat (Thoracic-10). Microdialysis perfusate (2 μL/min) was collected in the sample loop of an on-line injector for direct injection onto a glassy carbon electrode which was modified with the heat-treated single-walled carbon nanotubes (SWNTs). Normal ascorbic acid concentration in the extracellular fluids of spinal cords was (26.17 ±1.25) μmol/L (n=8). The experimental spinal cord injury, induced by a lesion at T-10, significantly increased the extracellular ascorbic acid levels to (53.24±1.95) μmol/L (n=8). This study provides the experimental evidence on the essential roles of ascorbic acid in spinal cord injuries.

Key words:

1. [1]
  Chehensse C, Bahrami S, Denys P, Clement P, Bernabe J, Giuliano F. Hum. Reprod. Update, 2013, 19(5):507-526
2. [2]
  Serarslan Y, Yonden Z, Ozgiray E. J. Clin. Neurosci., 2010, 17(3):349-352
3. [3]
  Tsai P J, Chen W Y, Tzeng S F, Liang W M, Yang C S. Clin. Chim. Acta, 2005, 362(1-2):94-100
4. [4]
  Yusa T. Brain Res.,2001, 897(1-2):104-113
5. [5]
  Szultka M, Buszewska-Forajta M, Kaliszan R, Buszewski B. Electrophoresis, 2014, 35(4):585-592
6. [6]
  Jin P, Xia L, Li Z, Che N, Zou D, Hu X. J. Pharm. Biomed. Anal., 2012, 70:151-157
7. [7]
  Zhu M, Zeng C, Ye J.Electroanalysis, 2011, 23(4):907-914
8. [8]
  Liu K, Yu P, Lin Y, Wang Y, Ohsaka T, Mao L. Anal Chem., 2013, 85(20):9947-9954
9. [9]
  Li L, Zhang Y, Hao J, Liu J, Yu P, Ma F, Mao L.Analyst, 2016, 141(7):2199-2207
10. [10]
  Tao L, Song C, Sun Y. Anal. Chim. Acta, 2013, 761:194-200
11. [11]
  Talac R, Friedman J A, Moore M J. Biomaterials, 2004, 25(9):1505-1510
12. [12]
  ZHANG Ren-Yan, ZHANG Xue-Ao, JIA Hong-Hui, LI Xin-Hua.Chinese J. Anal. Chem., 2012, 40(6):909-914 张仁彦, 张学骜, 贾红辉, 李新华. 分析化学, 2012, 40(6):909-914
13. [13]
  ZHANG Cui-Zhong, WANG Li-Wei, LU Yong-Ke, ZHENG Guang-Jin, PENG Jin-Yun. Chinese J. Anal. Chem., 2016, 44(8):1263-1269 张翠忠, 王丽伟, 卢永课, 郑广进, 彭金云. 分析化学, 2016, 44(8):1263-1269
14. [14]
  Barabas J, Nagy E, Degrell I. Arch. Gerontol. Geriatr., 1995, 21(1):43-48
15. [15]
  Liu D, Xu G Y, Pan E, McAdoo D J. Neuroscience, 1999, 93(4):1383-1389
16. [16]
  McAdoo D J, Xu G Y, Robak G, Hughes M G. Exp. Neurol., 1999, 159(2):538-544
17. [17]
  Xu G Y, Hughes M G, Ye Z, Hulsebosch C E, McAdoo D J. Exp. Neurol., 2004, 187(2):329-336
18. [18]
  McAdoo D J, Wu P. Pharmacol. Biochem. Behav., 2008, 90(2):282-296
19. [19]
  Thompson R J, Zhou N, MacVicar B A. Science, 2006, 312(5775):924-927

扫一扫看文章

计量

PDF下载量: 4
文章访问数: 496
HTML全文浏览量: 43

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

English

Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析

English

Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs