基于同步辐射X射线荧光光谱和X射线吸收近边结构谱研究铅锌矿区浮萍中砷的耐受机制

引用本文: 储彬彬, 罗立强, 马艳红. 基于同步辐射X射线荧光光谱和X射线吸收近边结构谱研究铅锌矿区浮萍中砷的耐受机制[J]. 分析化学, 2017, 45(5): 668-673. doi: 10.11895/j.issn.0253-3820.170025 shu

Citation: CHU Bin-Bin, LUO Li-Qiang, MA Yan-Hong. Study on Mechanism of Arsenic Tolerance in Duckweeds from Lead-Zinc Mine by Synchrotron Radiation X-ray Fluorescence and X-ray Absorption Near Edge Structure Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 668-673. doi: 10.11895/j.issn.0253-3820.170025 shu

基于同步辐射X射线荧光光谱和X射线吸收近边结构谱研究铅锌矿区浮萍中砷的耐受机制

国家地质实验测试中心, 北京 100037
基金项目:
本文系国家重点研发计划（No. 2016YFC0600605）、中国地质科学院基本科研业务费项目（No.YYWF201524）和中国地质调查局地质大调查项目（No.12120113015200）资助

摘要: 水生植物浮萍能够富集高浓度的砷，是植物过滤修复技术的代表，其耐受机理备受关注。本研究利用同步辐射X射线荧光光谱（Synchrotron radiation X-ray fluorescence，SRXRF）和X射线吸收近边结构谱（X-ray absorption near edge structure，XANES）技术，开展了铅锌矿区天然富砷浮萍样品中砷元素的微区分布和形态特征研究。微区SRXRF分析了两个单片浮萍样品FP1和FP2，单点扫描时间分别为5 s和2 s，光斑尺寸分别为70 μm×80 μm和100 μm×100 μm；对FP2样品选择了6个位置进行了微区XANES单点形态分析，扫描的能量范围为11.81-11.96 keV；整体XANES形态分析是针对压片的浮萍样品，扫描的能量范围为11.67-12.27 keV。结果表明，浮萍样品整体和微区XANES数据均表明砷形态为As（Ⅲ）；SRXRF微区分析发现浮萍中砷元素具有显著的叶脉分布特征，在一定浓度范围内，砷并不扩散到进行光合作用的叶肉中，有利于减轻砷对叶片的毒害作用，可能是浮萍耐受砷的一个保护机制。

关键词:

浮萍
/ 砷
/ 微区分布
/ 形态

English

Study on Mechanism of Arsenic Tolerance in Duckweeds from Lead-Zinc Mine by Synchrotron Radiation X-ray Fluorescence and X-ray Absorption Near Edge Structure Spectrometry

National Research Center for Geoanalysis, Beijing 100037, China
Received Date: 13 January 2017
Revised Date: 04 March 2017
Fund Project:
This work was supported by the Major State Basic Research Development Program (No. 2016YFC0600605).

Abstract: Aquatic plant duckweed can enrich high concentration of arsenic, it is thus used as the representative of phytofiltration. The mechanism of arsenic tolerance in duckweeds has received much concern. In this study, synchrotron radiation X-ray fluorescence (SRXRF) and X-ray absorption near edge structure (XANES) techniques were used to study the micro-distribution and speciation of arsenic in natural As-rich duckweed from lead-zinc mine. Two monolithic duckweeds, FP1 and FP2, were analyzed by micro SRXRF, setting single point scan time and spot size were 5 s, 70 μm×80 μm and 2 s, 100 μm×100 μm respectively. Six points of FP2 were selected and analyzed by micro XANES in energy range of 11.81-11.96 keV. Pressed-pellet duckweed was analyzed by bulk XANES in energy range of 11.67-12.27 keV. The result showed that As(Ⅲ) was the major speciation of duckweed from bulk XANES and micro-XANES data. SRXRF micro analysis showed that arsenic had significant vein distribution in duckweed, and was not spread into the photosynthetic mesophyll within certain concentration, which may reduce the leaf toxicity triggered by arsenic. This vein distribution may play a role in arsenic tolerance in duckweed.

Key words:

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基于同步辐射X射线荧光光谱和X射线吸收近边结构谱研究铅锌矿区浮萍中砷的耐受机制

English

Study on Mechanism of Arsenic Tolerance in Duckweeds from Lead-Zinc Mine by Synchrotron Radiation X-ray Fluorescence and X-ray Absorption Near Edge Structure Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于同步辐射X射线荧光光谱和X射线吸收近边结构谱研究铅锌矿区浮萍中砷的耐受机制

English

Study on Mechanism of Arsenic Tolerance in Duckweeds from Lead-Zinc Mine by Synchrotron Radiation X-ray Fluorescence and X-ray Absorption Near Edge Structure Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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