基于压电振荡原理的微阵列点样系统的研制

引用本文: 王伟, 刘振邦, 张国玉, 王振新, 韩冬雪, 牛利, 包宇. 基于压电振荡原理的微阵列点样系统的研制[J]. 分析化学, 2017, 45(4): 620-625. doi: 10.11895/j.issn.0253-3820.160928 shu

Citation: WANG Wei, LIU Zhen-Bang, ZHANG Guo-Yu, WANG Zhen-Xin, HAN Dong-Xue, NIU Li, BAO Yu. Development of Piezoelectric Oscillation- Based Microarray Spotting System[J]. Chinese Journal of Analytical Chemistry, 2017, 45(4): 620-625. doi: 10.11895/j.issn.0253-3820.160928 shu

基于压电振荡原理的微阵列点样系统的研制

王伟 ^1,2, ,
刘振邦 ^2, ,
张国玉 ^1, ,
王振新 ^2, ,
韩冬雪 ^2, ,
牛利 ^2, ,
包宇 ^2,

1.
长春理工大学光电工程学院, 长春 130022;
2.
中国科学院长春应用化学研究所, 长春 130022
基金项目:
本文系中国科学院科研装备研制项目（No.YZ201355）资助

摘要: 针对基于电磁微阀原理的非接触点样方式存在操作过程复杂、点样量偏大，以及压电喷墨原理的非接触点样方式存在点样针不易清洗、造价昂贵等不足，研制了一种基于压电振荡原理的新型非接触点样装置，实现了微量液体点样。在本装置中，毛细管点样针与压电驱动装置为两个独立单元，可以单独对毛细管点样针进行更换和清洗。采用激光拉制法制备的玻璃毛细管点样针具有内径可调、成本低等优点。此点样方式通过改变压电陶瓷的振幅和频率，可在10^-10~10^-9 L之间调控点样体积。以此为基础，结合三维精密位移控制技术，研制了一种基于压电振荡原理的微阵列生物芯片点样系统。对点样系统的点样体积、点样密度、点样精度等参数进行了测试，结果表明，此点样系统的最小点样体积可达320 pL，点样密度可达4000 点/cm²，并能够实现界面图案化制备。

关键词:

English

Development of Piezoelectric Oscillation- Based Microarray Spotting System

1.
The School of Photo-Electronic Engineering, Changchun University of Science and Technology, Changchun 130022, China;
2.
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Received Date: 28 December 2016
Revised Date: 06 February 2017

Abstract: A new type of piezoelectric oscillation-based non-contact spotting mode has been developed to overcome the disadvantages of traditional non-contact spotting modes including complicated operation procedure, cleaning difficulty of spotting needle, large sample consumption of electromagnetic microvalve and high spotting cost of piezoelectric inkjet based non-contact spotting mode. In the device, the capillary spotting needle and the piezoelectric driving device are two independent units used for replacing and cleaning capillary spotting needle. The glass capillary spotting needle is prepared by the laser melting method with adjustable diameter and low cost. The sample spotting volume of the device can be easily adjusted in the range of 10^-10-10^-9 by changing the amplitude and frequency of piezoelectric ceramic. A microarray spotting system is developed by the combination of the piezoelectric oscillation-based non-contact spotting mode and three dimensional precision displacement control technology. The multiple parameters of as-prepared microarray spotting system have been tested including spotting volume, density of spot and spotting precision. The experimental results indicate that the minimum volume of single spot with 320 pL and the highest density of spot with 4000 spots/cm² can be achieved by the as-prepared microarray spotting system. Furthermore, the as-prepared microarray spotting system can also be employed to fabricate patterned interface.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于压电振荡原理的微阵列点样系统的研制

English

Development of Piezoelectric Oscillation- Based Microarray Spotting System

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于压电振荡原理的微阵列点样系统的研制

English

Development of Piezoelectric Oscillation- Based Microarray Spotting System

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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