增强氯化血红素催化活性用于水胺硫磷比色检测研究

引用本文: 陈华云, 吴远根, 杨文平, 赵静. 增强氯化血红素催化活性用于水胺硫磷比色检测研究[J]. 分析化学, 2017, 45(4): 508-513. doi: 10.11895/j.issn.0253-3820.160875 shu

Citation: CHEN Hua-Yun, WU Yuan-Gen, YANG Wen-Ping, ZHAO Jing. Colorimetric Determination of Isocarbophos Based on Enhanced Peroxidase-like Activity of Hemin[J]. Chinese Journal of Analytical Chemistry, 2017, 45(4): 508-513. doi: 10.11895/j.issn.0253-3820.160875 shu

增强氯化血红素催化活性用于水胺硫磷比色检测研究

1.
贵州大学发酵工程与生物制药重点实验室, 贵阳 550025;
2.
贵州大学酿酒与食品工程学院, 贵阳 550025
基金项目:
本文系国家自然科学基金（Nos.21565009，21205020）、贵州省科学技术基金（黔科合基础[2016]1403）、贵州省科技厅科技合作计划（黔科合LH字[2015]7682）、贵州省教育厅招标项目（黔教合KY字[2014]261）、贵州大学引进人才科研项目（贵大人基合字（2014）09号）、贵州大学研究生创新基金（No.2016056）资助

摘要: 建立了一种基于增强氯化血红素（Hemin）过氧化物酶催化活性比色检测水胺硫磷的方法。在H₂O₂存在下，Hemin催化氧化底物3，3'，5，5'-四甲基联苯胺（TMB），使其失去1个电子，导致反应体系由无色变为蓝绿色。水胺硫磷的加入可提高Hemin对底物亲和力，进一步增强其催化活性，使底物氧化失去2个电子，反应体系由蓝绿色变为黄色，且颜色变化程度与水胺硫磷浓度成正比。在最优条件下，本方法的动态检测范围为2-100 μg/L，检出限为1.2 μg/L（3σ）。其它有机磷农药对水胺硫磷检测无明显干扰，实际样品中水胺硫磷的加标回收率为93.0%-113.0%。本方法可应用于农产品中水胺硫磷残留的检测。

关键词:

English

Colorimetric Determination of Isocarbophos Based on Enhanced Peroxidase-like Activity of Hemin

1.
Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang 550025, China;
2.
School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China
Received Date: 30 November 2016
Revised Date: 03 January 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21565009, 21205020).

Abstract: A simple colorimetric method for detection of isocarbophos was developed based on the enhanced peroxidase-like activity of hemin. Hemin could catalyze the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂, which made TMB to lose one electron and caused reaction solution color changing from initial transparent to blue-green. Adding isocarbophos improved hemin affinity to substrates, which further enhanced peroxidase-like activity of hemin and made TMB to lose two electrons. The color of TMB solution was further changed from blue-green to yellow, and the degree of color change was proportional to the concentration of isocarbophos. Under the optimal conditions, the present analytical method for isocarbophos detection had a dynamic range from 2 μg/L to 100 μg/L with a detection limit of 1.2 μg/L (3σ). The selectivity assay demonstrated that other organophosphorus pesticides exhibited negligible interferences for isocarbophos detection. The application of the proposed method in the practical samples showed that the mean recovery of isocarbophos was in the range of 94.4%-113.0%, thus it could be widely applied to rapidly and sensitively detect isocarbophos in the agricultural products.

Key words:

1. [1]
  Darko G, Akoto O. Food Chem. Toxicol., 2008, 46: 3703-3706
2. [2]
  Kumar V, Upadhyay N, Kumar V, Sharma S. Arab J. Chem., 2015, 8: 624-631
3. [3]
  ZHANG Can, ZHANG Jin-Xing, WANG Kun, DAI Zhi-Hui. Acta Chim. Sinica, 2012, 70(8): 1008-1012 张灿, 张金星, 王坤, 戴志晖. 化学学报, 2012, 70(8): 1008-1012
4. [4]
  Sun J, Dong T, Zhang Y, Wang S. Anal. Chim. Acta, 2010, 666: 76-82
5. [5]
  Watanabe E, Baba K, Eun H, Miyake S. Food Chem., 2007, 102: 745-750
6. [6]
  Zhang C, Wang L, Tu Z, Sun X, He Q, Lei Z, Xu C, Liu Y, Zhang X, Yang J, Liu X, Xu Y. Biosens. Bioelectron., 2014, 55: 216-219
7. [7]
  WANG Li, YE Hua, SANG Hong-Qing, WANG Dan-Dan. Chinese J. Anal. Chem., 2016, 44(5): 799-803 王丽, 叶华, 桑宏庆, 王丹丹. 分析化学, 2016, 44(5): 799-803
8. [8]
  Bala R, Kumar M, Bansal K, Sharma R K, Wangoo N. Biosens. Bioelectron., 2016, 85: 445-449
9. [9]
  Zhang S X, Xue S F, Deng J, Zhang M, Shi G, Zhou T. Biosens. Bioelectron., 2016, 85: 457-463
10. [10]
  Zheng L, Pi F, Wang Y, Xu H, Zhang Y, Sun X J. Hazard. Mater., 2016, 315: 11-22
11. [11]
  Fu J, Tan X H, Li Y H, Song X J. Chin. Chem. Lett., 2016, 27: 1541-1546
12. [12]
  SHEn Jing-Can, WU Feng-Qi, XIAo Chen-Gui, ZHANG Yi, HAN Rui-YANG, HONG Xiao-Liu. Chinese J. Appl. Chem., 2014, 31(1): 84-88 沈金灿, 吴凤琪, 肖陈贵, 张毅, 韩瑞阳, 洪小柳. 应用化学, 2014, 31(1): 84-88
13. [13]
  CHENG Hao, GAO Xiao-Guang, JIA Jian, LI Jian-Ping, HE Xiu-Li. Chinese J. Anal. Chem., 2010, 38(11): 1683-1686 程浩, 高晓光, 贾建, 李建平, 何秀丽. 分析化学, 2010, 38(11): 1683-1686
14. [14]
  Machado M F, Saraiva J. J. Mol. Catal. B, 2002, 19: 451-457
15. [15]
  Jo E J, Mun H, Kim S J, Shim W B, Kim M G. Food Chem., 2016, 194: 1102-1107
16. [16]
  Cheng F F, Jiang N, Li X, Zhang L, Hu L, Chen X, Jiang L P, Abdel-Halim E S, Zhu J J. Biosens. Bioelectron., 2016, 85: 891-896
17. [17]
  Zou P, Liu Y, Wang H, Wu J, Zhu F, Wu H. Biosens. Bioelectron., 2016, 79: 29-33
18. [18]
  Wu Y, Wang F, Zhan S, Liu L, Luo Y, Zhou P. RSC Adv., 2013, 3: 25614-25619
19. [19]
  Chen H, Wu Y, Yang W, Zhan S, Qiu S, Zhou P. Sens. Actuator B, 2017, 243: 445-453
20. [20]
  GAO Li-Zeng, YAN Xi-Yun. Prog. Biochem. Biophys., 2013, 40(10): 892-902 高利增, 阎锡蕴. 生物化学与生物物理进展, 2013, 40(10): 892-902
21. [21]
  Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X. Nat. Nanotechnol., 2007, 2: 577-583
22. [22]
  Wu Y, Zhan S, Wang F, He L, Zhi W, Zhou P. Chem. Commun., 2012, 48: 4459-4461
23. [23]
  Wu Y, Zhan S, Xu L, Shi W, Xi T, Zhan X, Zhou P. Chem. Commun., 2011, 47: 6027-6029

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

增强氯化血红素催化活性用于水胺硫磷比色检测研究

English

Colorimetric Determination of Isocarbophos Based on Enhanced Peroxidase-like Activity of Hemin

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

增强氯化血红素催化活性用于水胺硫磷比色检测研究

English

Colorimetric Determination of Isocarbophos Based on Enhanced Peroxidase-like Activity of Hemin

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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