基于化学指纹图谱和抗血小板聚集效价的丹参质量评价

引用本文: 刘振杰, 史志龙, 王伽伯, 张海珠, 李春雨, 牛明, 何琴, 甄汉深, 肖小河. 基于化学指纹图谱和抗血小板聚集效价的丹参质量评价[J]. 分析化学, 2017, 45(5): 693-699. doi: 10.11895/j.issn.0253-3820.160864 shu

Citation: LIU Zhen-Jie, SHI Zhi-Long, WANG Jia-Bo, ZHANG Hai-Zhu, LI Chun-Yu, NIU Ming, HE Qin, ZHEN Han-Shen, XIAO Xiao-He. Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 693-699. doi: 10.11895/j.issn.0253-3820.160864 shu

基于化学指纹图谱和抗血小板聚集效价的丹参质量评价

1.
解放军302医院全军中医药研究所, 北京 100039;
2.
广西药用植物园, 南宁 530023;
3.
中药资源系统研究与开发利用省部共建国家重点实验室培育基地, 成都 610075;
4.
广西中医药大学, 南宁 530001
基金项目:
本文系国家自然科学基金项目（No. 81274026）、国家中医药管理局中医药行业科研专项（No.201507002）、中药资源系统研究与开发利用省部共建国家重点实验室培育基地开放研究基金项目、十二五军队重点课题（No. BWS11J049）资助

摘要: 通过化学分析和生物活性评价考察丹参药材的品质差异，探讨丹参抗血小板聚集生物活性的主要贡献成分。采用高效液相色谱（HPLC）技术建立丹参药材HPLC指纹图谱，以抗血小板聚集相对效价作为指标，评价不同产地不同批次丹参药材的品质差异，构建基于化学表征及生物效价测定的评价模式。结果表明，不同批次丹参药材的HPLC指纹图谱相似度很高（相似度0.930~0.998），而其抗血小板聚集相对效价相差10倍，提示化学指纹图谱难以反映丹参的活性和质量差异。通过化学指纹图谱与抗血小板聚集生物效价进行谱效相关分析，筛选出与生物活性相关系数大于0.5的6个色谱峰：二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA及2个未知化合物。对上述4种已知化合物单体进行活性验证发现，隐丹参酮的抗血小板聚集活性最强，而其它3种丹参酮类化合物几乎没有体外抗血小板聚集活性。进一步比较丹参中高含量成分丹酚酸B与低含量成分隐丹参酮的活性贡献，结果表明，两者的活性贡献基本相当，说明隐丹参酮是丹参中低含量高活性成分，对评价丹参质量具有重要贡献度。

关键词:

English

Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation

1.
China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing 100039, China;
2.
Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;
3.
State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;
4.
College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China
Received Date: 26 November 2016
Revised Date: 03 March 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 81274026), the National Industry Science & Technology Foundation (No.201507002), the Open Research Fund of the State Key Laboratory Breeding Base of Systematic Research, and the Development and Utilization of Chinese Medicinal Resources and the Key Army Project (No.BWS11J049).

Abstract: Chemical analysis and bioassay were used to distinguish quality grades of Salvia miltiorrhiza bge., and the active compounds were investigated on anti-platelet aggregation. We established the HPLC fingerprint of Salvia miltiorrhiza bge. and meansured thir biopotency based on anti-platelet aggregation. The results showed that the HPLC fingerprints of different batches of Salvia miltiorrhiza bge. were similar (Similarity=0.930-0.998), but the difference of biopotency could be ten-fold. Through correlation analysis of chemical fingerprint and anti-platelet aggregation assay, dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ, tanshinone ⅡA, and two unknown compounds were closely related to the biopotency. It was proved that cryptotanshinone was a high activity component on platelet aggregation. To compare the active contribution of the high content Salvianolic acid B and low content of the active ingredient cryptotanshinone, the active contribution value of the two compounds were basically equivalent. Cryptotanshinone, which had low content but high activity, made an important contribution to evaluating the quality of Salvia miltiorrhiza bge.. The results indicated that the quality of traditional Chinese medicine could not be evaluated accurately by only using the chemical content or abundance. This work established a quality evaluation model based on chemical fingerprint and bioassay which could improve the quality evaluation of traditional Chinese medicine associating with effects.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于化学指纹图谱和抗血小板聚集效价的丹参质量评价

English

Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于化学指纹图谱和抗血小板聚集效价的丹参质量评价

English

Quality Evaluation of Salvia Miltiorrhiza Bge. Based on Chemical Profile and Bioassay of Anti-platelet Aggregation

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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