基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响

引用本文: 徐盼盼, 宋悦, 陈娟娟, 杨锐, 严小军, 钟琪. 基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响[J]. 分析化学, 2017, 45(5): 641-647. doi: 10.11895/j.issn.0253-3820.160748 shu

Citation: XU Pan-Pan, SONG Yue, CHEN Juan-Juan, YANG Rui, YAN Xiao-Jun, ZHONG Qi. Effects of Fucoidan on Lipidomics Profiling of Juvenile Yellow Catfish (Pelteobagrus fulvidraco) during Feeding Time[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 641-647. doi: 10.11895/j.issn.0253-3820.160748 shu

基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响

徐盼盼 ^1, ,
宋悦 ^1, ,
陈娟娟 ^1, ,
杨锐 ^1, ,
严小军 ^2, ,
钟琪 ^1,

1.
浙江省海洋生物工程重点实验室, 宁波大学, 宁波 315211;
2.
李达三叶耀珍伉俪李本俊海洋生物医药研究中心, 宁波大学, 宁波 315211
基金项目:
本文系浙江省自然科学基金（No. LY17D060002）；浙江省科技厅公益项目（分析测试）（No. 2016C37090），宁波大学李达三叶耀珍伉俪李本俊海洋生物医药发展基金，国家111引智基地和宁波大学王宽诚幸福基金资助

摘要: 采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF-MS）技术，结合多元变量数据分析方法，研究0.1%褐藻糖胶喂养黄颡鱼幼鱼1~8周的脂质代谢组学。通过不同喂养时间的黄颡鱼脂质成分的重要性（VIP）值和差异性（P）值，筛选出98个差异性代谢物，其中鉴定出与褐藻糖胶对黄颡鱼随喂养时间变化的影响有重要作用的11种脂质生物标志物，包括Lyso-PC 16∶0、PC 22∶6/16∶0、PE 22∶6/16∶0、PI 18∶0/22∶6、DAG 16∶0/16∶0、DAG 16∶0/18∶1、DAG 18∶1/18∶1、TAG 20∶5/16∶0/18∶3、TAG 20∶4/16∶1/18∶2、TAG 16∶0/18∶2/20∶5 和TAG 18∶2/14∶0/18∶1。研究表明，经0.1%褐藻糖胶喂养不同时期的黄颡鱼中Lyso-PC、PC、PE和PI的含量在第8周时达到最大值；DAG的含量呈现出先减少后增加的变化规律，而TAG的变化不定，TAG 18∶2/14∶0/18∶1、TAG 20∶5/16∶0/18∶3和TAG 20∶4/16∶1/18∶2的含量随着喂养褐藻糖胶时间的延长而增加，而TAG 16∶0/18∶2/20∶5呈减少的趋势。根据上述实验结果，推测褐藻糖胶可能通过调节脂质成分的含量来增加黄颡鱼幼鱼的免疫力。本研究结果为褐藻糖胶对黄颡鱼脂质代谢响应机理的影响的研究提供理论依据。

关键词:

English

Effects of Fucoidan on Lipidomics Profiling of Juvenile Yellow Catfish (Pelteobagrus fulvidraco) during Feeding Time

1.
Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China;
2.
Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center at Ningbo University, Ningbo 315211, China
Received Date: 09 October 2016
Revised Date: 06 March 2017
Fund Project:
This work was supported by the Natural Science Foundation of Zhejiang Province, China (No.LY17D060002), the Science Technology Department of Zhejiang Province (No. 2016C37090), Li Dak Sum Yip Yio Chin Kenneth LiMarine Biopharmaceutical Development Fund, National 111 Project of China and K.C. Wong Magna Fund in Ningbo University.

Abstract: The effects of fucoidan on the lipidomics profiling of juvenile yellow catfish (pelteobagrus fulvidraco) during different feeding time (week 1, week 2, week 3 and week 8) were investigated by ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry (UPLC-Q-TOF-MS) combined with multivariate data analysis, e.g. principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA). Based on VIP and p values, 11 lipid biomarkers were screened including lysophosphatidylcholine (Lyso-PC) 16∶0, phosphatidylcholine (PC) 22∶6/16∶0, phosphatidylethanolamine (PE) 22∶6/16∶0, phosphatidylinositol (PI) 18∶0/22∶6, diacylglycerol (DAG) 16∶0/16∶0,DAG 16∶0/18∶1, DAG 18∶1/18∶1, triglycerides (TAG) 20∶5/16∶0/18∶3, TAG 20∶4/16∶1/18∶2, TAG 16∶0/18∶2/20∶5 and TAG 18∶2/14∶0/18∶1. It was found that Lyso-PC, PC, PE and PI had the largest levels at the eighth week; the levels of DAG were decreased at the second and fourth weeks, and increased at the eighth week. While TAG was different: the content of TAG 18∶2/14∶0/18∶1, TAG 20∶5/16∶0/18∶3 and TAG 20∶4/16∶1/18∶2 increased in response to fucoidan, but TAG 16∶0/18∶2/20∶5 decreased little. Therefore, for juvenile yellow catfish, fucoidan can affect its lipid metabolism, which provides a theoretical basis for investigation of the influence of fucoidan on the response mechanism of lipid metabolism of juvenile yellow catfish.

Key words:

Pelteoobagrus fulvidraco
/ Fucoidan
/ Ultra-performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry
/ Lipidomic

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基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响

English

Effects of Fucoidan on Lipidomics Profiling of Juvenile Yellow Catfish (Pelteobagrus fulvidraco) during Feeding Time

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基于脂质代谢组学研究褐藻糖胶对黄颡鱼幼鱼的影响

English

Effects of Fucoidan on Lipidomics Profiling of Juvenile Yellow Catfish (Pelteobagrus fulvidraco) during Feeding Time

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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