磷脂微乳电动色谱的定量结构保留关系研究

引用本文: 宋静, 郑园, 霍长虹, 刘建芳. 磷脂微乳电动色谱的定量结构保留关系研究[J]. 分析化学, 2017, 45(5): 654-661,753. doi: 10.11895/j.issn.0253-3820.160732 shu

Citation: SONG Jing, ZHENG Yuan, HUO Chang-Hong, LIU Jian-Fang. Study on Quantitative Structure-Retention Relationships of Microemulsion Electrokinetic Chromatography Using Phospholipids as Surfactant[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 654-661,753. doi: 10.11895/j.issn.0253-3820.160732 shu

磷脂微乳电动色谱的定量结构保留关系研究

宋静 ^1, ,
郑园 ^2, ,
霍长虹 ^1, ,
刘建芳 ^1,3,

1.
河北医科大学研究生学院, 石家庄 050017;
2.
天津医科大学总医院, 天津 300052;
3.
白求恩国际和平医院, 石家庄 050082
基金项目:
本文系国家自然科学基金项目（No. 81202501）资助

摘要: 以大豆磷脂为主要的表面活性剂，制备适合毛细管电动色谱使用的不同构成比的微乳体系，应用溶剂化参数模型研究了中性溶质在其中的定量结构保留关系。使用动态涂层毛细管，以二甲基亚砜和十二烷基苯分别作为电渗流和微乳液滴迁移的标记物，测定了26个具有不同结构小分子中性化合物在17种微乳电动色谱体系下的保留因子，建立了线性溶剂化能量关系（LSER）方程。通过比较两体系的LSER方程系数比较体系相似性。结果表明，本研究建立的磷脂微乳电动色谱体系在线性溶剂化特征上和其它构成的微乳电动色谱体系相似。对溶质保留贡献较大的是溶质体积和有效氢键碱度，油相种类及浓度对溶质的保留选择性无明显影响。

关键词:

English

Study on Quantitative Structure-Retention Relationships of Microemulsion Electrokinetic Chromatography Using Phospholipids as Surfactant

1.
Department of Graduate School, Hebei Medical University, Shijiazhuang 050017, China;
2.
Department of Pharmacy, General Hospital of Tianjin Medical University, Tianjin 300052, China;
3.
Department of Pharmacy, Bethune International Peace Hospital, Shijiazhuang 050082, China
Received Date: 28 September 2016
Revised Date: 20 January 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 81202501)

Abstract: Some new types of microemulsion using phospholipids as the main surfactant were prepared for electrokinetic chromatography and the quantitative structure-retention relationship of neutral solutes in these microemulsion electrokinetic chromatography (MEEKC) systems was studied by solvation parameters model. By using dynamic coating capillary, and with dimethyl sulfoxide (DMSO) and dodecyl benzene as the marker of electroosmotic flow and microemulsion droplets, a total of 17 kinds of stable microemulsions containing soybean phospholipids or other surfactants were prepared and the linear salvation energy relationship equations were developed for these MEEKC systems with 26 small neutral compounds. The coefficients of linear solvation energy relationship (LSER) equations were used to evaluate the similarity of two MEEKC systems. Results indicated that LSER characteristics of phospholipids-MEEKC systems were similar to those of other microemulsion systems. The volume and hydrogen bond basicity of solutes were mostly contributed to the retention in MEEKC. The different types and concentration of oil phase had no evident influence on the retention.

Key words:

Microemulsion electrokinetic chromatography
/ Soybean phospholipids
/ Retention indice
/ Linear solvation energy relationship

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沈阳化工大学材料科学与工程学院沈阳 110142

磷脂微乳电动色谱的定量结构保留关系研究

English

Study on Quantitative Structure-Retention Relationships of Microemulsion Electrokinetic Chromatography Using Phospholipids as Surfactant

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中国化学会秘书处

磷脂微乳电动色谱的定量结构保留关系研究

English

Study on Quantitative Structure-Retention Relationships of Microemulsion Electrokinetic Chromatography Using Phospholipids as Surfactant

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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