基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

引用本文: 吴亮亮, 钱志娟, 谢正军, 张莹莹, 彭池方. 基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究[J]. 分析化学, 2017, 45(4): 471-476. doi: 10.11895/j.issn.0253-3820.160710 shu

Citation: WU Liang-Liang, QIAN Zhi-Juan, XIE Zheng-Jun, ZHANG Ying-Ying, PENG Chi-Fang. Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme[J]. Chinese Journal of Analytical Chemistry, 2017, 45(4): 471-476. doi: 10.11895/j.issn.0253-3820.160710 shu

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

1.
江南大学, 食品科学与技术国家重点实验室, 食品学院, 无锡 214122;
2.
扬州出入境检验检疫局, 扬州 225000
基金项目:
本文系国家自然科学基金（No.31371767）、十二五国家科技支撑计划项目（No.2015BAD17B02）和江苏省出入境检验检疫局科技项目（No.2017KJ19）资助

摘要: 通过对银/铂纳米簇（Ag/Pt NCs）的表面修饰调控其催化活性，建立了一种高灵敏的比色法检测Cu²⁺。巯基丙酸能够抑制Ag/Pt NCs的催化活性，而巯基丙酸与Cu²⁺作用后，将导致上述抑制作用减弱。基于上述原理，通过测量Ag/Pt NCs 催化TMB-H₂O₂反应产生的显色信号，可以实现Cu²⁺的比色检测。本方法检测Cu²⁺的线性范围为10~100 nmol/L，检出限（3σ）为5.0 nmol/L。将本方法应用于实际水样中Cu²⁺的检测，结果表明，本方法具有操作简单、成本低、灵敏度高、特异性好等优点。

关键词:

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

1.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2.
Yangzhou Entry-Exit Inspection and Quarantine Bureau, Yangzhou 225000, China
Received Date: 21 September 2016
Revised Date: 21 November 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.31371767) and the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (No.2015BAD17B02).

Abstract: A sensitive colorimetric method for the detection of copper ions (Cu²⁺) was developed based on the surface modification of silver/platinum nanoclusters (Ag/Pt NCs) and regulation of peroxidase-like activity. It was found that 3-mercaptopropionic acid (MPA) could inhibit the catalytic ability of Ag/Pt NCs; however, it lost the inhibition toward catalytic ability of Ag/Pt NCs after oxidized by oxygen through the catalysis of Cu²⁺. On the basis of this, a colorimetric method was developed for the detection of Cu²⁺ through measuring the colorimetric signal variation of the TMB-H₂O₂ reaction. This method exhibited high sensitivity and selectivity toward Cu²⁺ over a panel of other metal ions. The linear range was 10-100 nmol/L and the detection limit was 5.0 nmol/L (3σ). The above method was also applied to detect real water samples and spiked samples, and the results demonstrated that this method was simple with low cost.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于银/铂纳米模拟酶表面修饰的铜离子比色检测方法研究

English

Colorimetric Detection of Copper Ions Based on Surface Modification of Silver/Platinum Cluster Nanoenzyme

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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