基于光波导微环谐振器的lgG非标记检测微流体分析系统

引用本文: 郭盈盈, 吴尚犬, 金里, 王皖君, 张青川. 基于光波导微环谐振器的lgG非标记检测微流体分析系统[J]. 分析化学, 2017, 45(4): 483-488. doi: 10.11895/j.issn.0253-3820.160650 shu

Citation: GUO Ying-Ying, WU Shang-Quan, JIN Li, WANG Wan-Jun, ZHANG Qing-Chuan. Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels[J]. Chinese Journal of Analytical Chemistry, 2017, 45(4): 483-488. doi: 10.11895/j.issn.0253-3820.160650 shu

基于光波导微环谐振器的lgG非标记检测微流体分析系统

1.
中国科学院材料力学行为和设计重点实验室, 中国科学技术大学近代力学系, 合肥 230027;
2.
中国电子技术集团公司第三十八研究所, 合肥 230001
基金项目:
本文系中国国际科技合作项目（No.2013DFE13090），国家自然科学基金项目（Nos.1162780010，11502265，11472266）资助

摘要: 设计开发了与微环谐振器集成的微流体通道系统，不仅避免了敞开环境中由于液体挥发造成的微环谐振器表面盐分的聚结，屏蔽空气中的各种杂质，而且只需要30 μL反应溶液，减少了药品用量，大大节约了实验成本。同时，采用绝缘体硅（SOI）材料，利用光刻技术设计和制作了波导宽度为450 nm，半径为5 μm，品质因子（Q值）为20000的光波导微环谐振器。集成的微环谐振器传感系统具有低成本、免标记、能实时监测生化反应过程等特点。以不同浓度的酒精溶液为测试对象，研究了微环谐振器对均质溶液的传感性能，传感芯片对溶液折射率的探测灵敏度为76.09 nm/RIU，探测极限为5.25×10^-4 RIU，验证了此微环谐振器对均质溶液进行浓度检测的可行性。利用此传感系统对人免疫球蛋白IgG进行了非标记免疫检测。在测试中，采用微流体通道系统将相应抗体修饰到微环谐振器表面，利用光谱仪对修饰过程以及抗原抗体特异性结合过程中的共振谱线漂移情况进行了监测。结果表明，光波导微环谐振器可以对生物分子进行实时监测。

关键词:

English

Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels

1.
Chinese Academy of Sciences, Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei 230027, China;
2.
China Electronics Technology Group Corporation No.38 Research Institute, Hefei 230001, China
Received Date: 02 September 2016
Revised Date: 15 February 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 1162780010, 11502265, 11472266)

Abstract: The microfluidic channels integrated with microring resonator were designed. The salt coalescence on chip surface caused by liquid volatilization in open environment was avoided and only 30 μL of reaction solution was consumed. These channels significantly reduced the experiment cost. The design, fabrication and characterization of a highly sensitive and label-free silicon-on-insulator (SOI) microring optical resonator integrated with the microfluidic channels were demonstrated. The radius of the microring was 5 μm and the straight waveguide with a width of 450 nm was employed in the microring resonator. The microring resonator device had many advantages such as high sensitivity, label-free and real-time detection. Using different concentrations of ethanol solution with known refractive indices, the refractive index detection sensitivity was 76.09 nm/RIU and the volume refractive index detection limit was 5.25×10^-4 RIU. We also demonstrated the label-free quantitative specific detections of human immunoglobulin G (IgG) solutions using an antibody-modified microring resonator by measuring the resonance wavelength shift resulting from refraction index changes causing by the immobilization of antibodies and specific recognition between antibodies and antigens, respectively. The results showed that the microring optical resonator could real-time monitor the reaction between biological molecules, the resonator could be used in the quantitative detection and biological sensing.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于光波导微环谐振器的lgG非标记检测微流体分析系统

English

Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于光波导微环谐振器的lgG非标记检测微流体分析系统

English

Label-Free Detection of Immunoglobulin G Based On Silicon-on-Insulator Microring Resonator Analysis System Integrated with Microfluidic Channels

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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