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Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS

Hong Kan ,  Wen-Yue Jiang ,  Ru Ding ,  Zhong-Ying Liu ,  Zi-Feng Pi ,  Zhi-Qiang Liu

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Citation:  Hong Kan, Wen-Yue Jiang, Ru Ding, Zhong-Ying Liu, Zi-Feng Pi, Zhi-Qiang Liu. Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS[J]. Chinese Chemical Letters, 2015, 26(5): 590-594. doi: 10.1016/j.cclet.2014.12.008 shu

Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS

    • 通讯作者: Zhong-Ying Liu,
    Zi-Feng Pi,
  • 基金项目:

    This work was supported by the National Natural Sciences Foundation of China (No. 81274046)  (No. 81274046)

    National Basic Research Program of China ("973 Program") (Nos. 2011CB505300, 2011CB505305). ("973 Program")

摘要: In this study, seven alkaloids were detected in Wu-tou decoction using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MSn). The aim of this study was to investigate the effect of Fritillariae Cirrhosae Bulbus, Fritillariae Thunbergii Bulbus, Pinelliae Rhizoma in different ratios with Wu-tou decoction (2:1, 1:1, 1:2) by measuring the therapeutic effects in Wu-tou decoction of main seven alkaloids including benzoylaconitine (BA), benzoylmesaconitine (BM), benzoylhypaconitine (BH), hypaconitine (HA), fuziline (FU), niaolin (NE) and deoxyaconitine (DA). The permeability of aconitum alkaloids extract through a Caco-2 cell monolayer was analyzed in the absence and presence of Fritillariae Cirrhosae Bulbus, Fritillariae Thunbergii Bulbus, and Pinelliae Rhizoma, respectively. The results showed that Pinelliae Rhizoma could reduce the absorption of the alkaloids and increase the excretion of the alkaloids, which would attenuate the therapeutic effects of Wu-tou decoction. Therefore, Pinelliae Rhizoma is an incompatible herb of Wu-tou decoction because of the inhibition of the absorption of alkaloids in the intestine. And that Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus showed the effects to improve the permeability of the alkaloids in Wu-tou decoction. These effects of these two herbs were similar, but the former was stronger than the latter, which most likely is due to the fact that the compositions of these two traditional Chinese medicines are similar. The in vitro data suggests that the compounds such as fritillary presented in alkaloids in the formula maybe improve the therapeutic function caused by the increased bioavailability of alkaloids in intestine.

English

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    1. [1] S.Z. Chen, Effect of Wu Tou Decoction on Rheumatoid Arthritis and its Possible Mechanism, (Master’s thesis), Guangzhou University of Traditional Chinese Medicine, Guangzhou, 2006.[1] S.Z. Chen, Effect of Wu Tou Decoction on Rheumatoid Arthritis and its Possible Mechanism, (Master’s thesis), Guangzhou University of Traditional Chinese Medicine, Guangzhou, 2006.

    2. [2] P.J. Wang, X.G. Shi, Z. Ge, X.D. Peng, Z.S. Huang, Effects of aconite decoction on peripheral blood T lymphocytes of adjuvant arthritis rat, Pharm. Clin. Chin. Mater. 23 (2007) 9-10.[2] P.J. Wang, X.G. Shi, Z. Ge, X.D. Peng, Z.S. Huang, Effects of aconite decoction on peripheral blood T lymphocytes of adjuvant arthritis rat, Pharm. Clin. Chin. Mater. 23 (2007) 9-10.

    3. [3] Y.Q. Sun, Effect and Mechanism of Aconite Decoction on Rheumatoid Arthritis, (Master’s thesis), China Academy of Chinese Medical Sciences, Beijing, 2012.[3] Y.Q. Sun, Effect and Mechanism of Aconite Decoction on Rheumatoid Arthritis, (Master’s thesis), China Academy of Chinese Medical Sciences, Beijing, 2012.

    4. [4] Y. Qi, S.Z. Li, Z.F. Pi, et al., Metabonomic study of Wu-tou decoction in adjuvantinduced arthritis rat using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, J. Chromatogr. B 953-954 (2014) 11-19.[4] Y. Qi, S.Z. Li, Z.F. Pi, et al., Metabonomic study of Wu-tou decoction in adjuvantinduced arthritis rat using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, J. Chromatogr. B 953-954 (2014) 11-19.

    5. [5] L. Wang, G.B. Zhou, P. Liu, et al., Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia, Proc. Natl. Acad. Sci. U.S.A. 105 (2008) 4826-4831.[5] L. Wang, G.B. Zhou, P. Liu, et al., Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia, Proc. Natl. Acad. Sci. U.S.A. 105 (2008) 4826-4831.

    6. [6] J.A. Duan, S.L. Su, X.S. Fan, et al., Interactions of seven emotions and the opposite/mutual inhibition/phase fear mode and mechanism of action and compatibility taboo based on traditional Chinese medicine drug, World Sci. Technol./Mod. Tradit. Chin. Med. Mater. Med. 14 (2012) 1547-1552.[6] J.A. Duan, S.L. Su, X.S. Fan, et al., Interactions of seven emotions and the opposite/mutual inhibition/phase fear mode and mechanism of action and compatibility taboo based on traditional Chinese medicine drug, World Sci. Technol./Mod. Tradit. Chin. Med. Mater. Med. 14 (2012) 1547-1552.

    7. [7] C. Wang, Y.G. Wang, Q.D. Liang, W.Q. Rang, Y. Gao, Analysis of chemical composition in the combination of monkshood and pinellia by UPLC/QTOFMS with multivariate statistical analysis, Acta Pharm. Sin. 45 (2010) 1301-1306.[7] C. Wang, Y.G. Wang, Q.D. Liang, W.Q. Rang, Y. Gao, Analysis of chemical composition in the combination of monkshood and pinellia by UPLC/QTOFMS with multivariate statistical analysis, Acta Pharm. Sin. 45 (2010) 1301-1306.

    8. [8] C. Wang, Y.G. Wang, Q.D. Liang, et al., Analysis of chemical composition in combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with multivariate statistical analysis, Acta Chim. Sin. 69 (2011) 1920-1928.[8] C. Wang, Y.G. Wang, Q.D. Liang, et al., Analysis of chemical composition in combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with multivariate statistical analysis, Acta Chim. Sin. 69 (2011) 1920-1928.

    9. [9] S.Y. Yee, In vitro permeability across Caco-2 cells (colonic) can predict in vivo (small intestinal) absorption in man - fact or myth, Pharm. Res. 14 (1997) 763-766.[9] S.Y. Yee, In vitro permeability across Caco-2 cells (colonic) can predict in vivo (small intestinal) absorption in man - fact or myth, Pharm. Res. 14 (1997) 763-766.

    10. [10] L. Ye, X.S. Yang, Z. Yang, et al., The role of efflux transporters on the transport of highly toxic aconitine, mesaconitine, hypaconitine, and their hydrolysates, as determined in cultured Caco-2 and transfected MDCKII cells, Toxicol. Lett. 216 (2013) 86-99.[10] L. Ye, X.S. Yang, Z. Yang, et al., The role of efflux transporters on the transport of highly toxic aconitine, mesaconitine, hypaconitine, and their hydrolysates, as determined in cultured Caco-2 and transfected MDCKII cells, Toxicol. Lett. 216 (2013) 86-99.

    11. [11] N. Li, R. Tsao, Z.G. Sui, et al., Intestinal transport of pure diester-type alkaloids from an aconite extract across the Caco-2 cell monolayer model, Planta Med. 78 (2012) 692-697.[11] N. Li, R. Tsao, Z.G. Sui, et al., Intestinal transport of pure diester-type alkaloids from an aconite extract across the Caco-2 cell monolayer model, Planta Med. 78 (2012) 692-697.

    12. [12] N.M. Akhir, L.S. Chua, F.A.A. Majid, M.R. Sarmidi, Cytotoxicity of aqueous and ethanolic extracts of Ficus deltoidea on human ovarian carcinoma cell line, Br. J. Med. Med. Res. 1 (2011) 397-409.[12] N.M. Akhir, L.S. Chua, F.A.A. Majid, M.R. Sarmidi, Cytotoxicity of aqueous and ethanolic extracts of Ficus deltoidea on human ovarian carcinoma cell line, Br. J. Med. Med. Res. 1 (2011) 397-409.

    13. [13] W. Wei, Studies on chemical constituents and bio-activities of degradation products of aconitines, (Ph.D. thesis), Jilin University, Changchun, 2011.[13] W. Wei, Studies on chemical constituents and bio-activities of degradation products of aconitines, (Ph.D. thesis), Jilin University, Changchun, 2011.

    14. [14] H.B. Zhu, Studies on Quality Control and Bioactivity Evaluation Methods of Radix aconiti Using Mass Spectrometry, (Ph.D. thesis), University of Chinese Academy of Sciences, Changchun, 2014.[14] H.B. Zhu, Studies on Quality Control and Bioactivity Evaluation Methods of Radix aconiti Using Mass Spectrometry, (Ph.D. thesis), University of Chinese Academy of Sciences, Changchun, 2014.

    15. [15] K. Fang, Z.H. Ling, G.H. Li, S.T. Shao, Protective effect of hypaconitine on rats’ cardiac muscle cell apoptosis induced by H2O2, Chin. J. Tradit. Med. Sci. Technol. 17 (2010) 315-317.[15] K. Fang, Z.H. Ling, G.H. Li, S.T. Shao, Protective effect of hypaconitine on rats’ cardiac muscle cell apoptosis induced by H2O2, Chin. J. Tradit. Med. Sci. Technol. 17 (2010) 315-317.

    16. [16] L. Xiong, C. Peng, X.F. Xie, et al., Alkaloids isolated from the lateral root of Aconitum carmichaelii, J. Mol. 17 (2012) 9939-9946.[16] L. Xiong, C. Peng, X.F. Xie, et al., Alkaloids isolated from the lateral root of Aconitum carmichaelii, J. Mol. 17 (2012) 9939-9946.

    17. [17] D.H. Xu, F.R. Song, H.F. Zhao, et al., Research of active components in Renshensili recipe, China J. Tradit. Chin. Med. Pharm. 17 (2002) 406-408.[17] D.H. Xu, F.R. Song, H.F. Zhao, et al., Research of active components in Renshensili recipe, China J. Tradit. Chin. Med. Pharm. 17 (2002) 406-408.

    18. [18] X.W. Yang, The Alkaloids, Chemical Industry Press, Beijing, 2005, pp. 357-358.[18] X.W. Yang, The Alkaloids, Chemical Industry Press, Beijing, 2005, pp. 357-358.

    19. [19] J.Z. Wang, G.Y. Han, Studies on alkaloids isolated from Jiangyou Fu Zi (Aconitum carmichaeli Debx), Acta Pharm. Sin. 20 (1985) 71-73.[19] J.Z. Wang, G.Y. Han, Studies on alkaloids isolated from Jiangyou Fu Zi (Aconitum carmichaeli Debx), Acta Pharm. Sin. 20 (1985) 71-73.

    20. [20] D.J. Song, M.W. Lu, H.Q. Li, et al., Anti-inflammatory, analgesic and antipyretic effects of deoxyacontine, Chin. Pharm. Bull. 3 (1987) 157-161.[20] D.J. Song, M.W. Lu, H.Q. Li, et al., Anti-inflammatory, analgesic and antipyretic effects of deoxyacontine, Chin. Pharm. Bull. 3 (1987) 157-161.

    21. [21] K.W. Hu, H.X. Zheng, J. Qi, et al., Empirical studies of peimine on reversing multidrug resistance of leukemia cells, Chin. J. Hematol. 20 (1999) 650-651.[21] K.W. Hu, H.X. Zheng, J. Qi, et al., Empirical studies of peimine on reversing multidrug resistance of leukemia cells, Chin. J. Hematol. 20 (1999) 650-651.

    22. [22] J.W. Ma, H. Kan, Y.H. Ma, et al., Qualitative and quantitative analysis of drug interactions: Fritillary mediating the transport of alkaloids in Caco-2 cells by P-glycoprotein, Chem. Res. Chin. Univ. 30 (2014) 731-737.[22] J.W. Ma, H. Kan, Y.H. Ma, et al., Qualitative and quantitative analysis of drug interactions: Fritillary mediating the transport of alkaloids in Caco-2 cells by P-glycoprotein, Chem. Res. Chin. Univ. 30 (2014) 731-737.

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  • 发布日期:  2014-12-16
  • 收稿日期:  2014-09-10
  • 网络出版日期:  2014-12-01
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