Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol

Ying Wang Di Zhang Fu Rong He Xiao Chun Chen

引用本文: Ying Wang,  Di Zhang,  Fu Rong He,  Xiao Chun Chen. Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol[J]. Chinese Chemical Letters, 2012, 23(2): 197-200. doi: 10.1016/j.cclet.2011.10.011 shu
Citation:  Ying Wang,  Di Zhang,  Fu Rong He,  Xiao Chun Chen. Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol[J]. Chinese Chemical Letters, 2012, 23(2): 197-200. doi: 10.1016/j.cclet.2011.10.011 shu

Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol

  • 基金项目:

    This work was supported by the National Natural Science Foundation of China (Nos. 21177013 and 50708007), the National Basic Research Program of China (973 Project, No. 2010CB429003), the Fundamental Research Funds for the Central Universities and special fund of State Key Laboratory of Water Environment Simulation (No. 11K04ESPCN).

摘要: Magnetic Cu2+-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination. The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol (PCP) degradation were evaluated systemically. Compared with free laccase, the immobilized laccase showed improved acid adaptability and thermal stability. The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.

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  • 收稿日期:  2011-05-27
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