引用本文:
Qi Liang Deng, Yan Li Li, Li Hua Zhang, Yu Kui Zhang. Molecularly imprinted macroporous monolithic materials for protein recognition[J]. Chinese Chemical Letters,
2011, 22(11): 1351-1354.
doi:
10.1016/j.cclet.2011.05.044
Citation: Qi Liang Deng, Yan Li Li, Li Hua Zhang, Yu Kui Zhang. Molecularly imprinted macroporous monolithic materials for protein recognition[J]. Chinese Chemical Letters, 2011, 22(11): 1351-1354. doi: 10.1016/j.cclet.2011.05.044
Citation: Qi Liang Deng, Yan Li Li, Li Hua Zhang, Yu Kui Zhang. Molecularly imprinted macroporous monolithic materials for protein recognition[J]. Chinese Chemical Letters, 2011, 22(11): 1351-1354. doi: 10.1016/j.cclet.2011.05.044
Molecularly imprinted macroporous monolithic materials for protein recognition
摘要:
Synthetic materials that can specifically recognize proteins will find wide application in many fields. In this report, bovine serum albumin was chosen as the template protein. Acrylamide and N, N'-methylenebisacrylamide were employed as the functional and cross-linker monomers, respectively. Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method. The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials. The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein, as compared with the control protein (hemoglobin), whereas the non-imprinted polymer (prepared under the same conditions except without addition template protein) had no selective properties.
English
Molecularly imprinted macroporous monolithic materials for protein recognition
Abstract:
Synthetic materials that can specifically recognize proteins will find wide application in many fields. In this report, bovine serum albumin was chosen as the template protein. Acrylamide and N, N'-methylenebisacrylamide were employed as the functional and cross-linker monomers, respectively. Molecularly imprinted macroporous monolithic materials that can preferentially bind the template protein in an aqueous environment were prepared by combination of molecular imprinting technique and freezing/thawing preparation method. The resulted imprinted macroporous monolithic columns were evaluated by utilizing as stationary phase in high performance liquid chromatography and solid-phase extraction materials. The experimental results indicated that the imprinted macroporous monolithic column exhibited good recognition for template protein, as compared with the control protein (hemoglobin), whereas the non-imprinted polymer (prepared under the same conditions except without addition template protein) had no selective properties.
扫一扫看文章
计量
- PDF下载量: 0
- 文章访问数: 593
- HTML全文浏览量: 4
下载:
