引用本文:
Ai Hua Fu, Zhu Jun Zhang, Li Li Chen, Xiao Ming Zhang, Pan Xue. High performance liquid chromatography with immobilized Ru(bpy)32+-KMnO4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes[J]. Chinese Chemical Letters,
2011, 22(10): 1245-1248.
doi:
10.1016/j.cclet.2011.05.023
Citation: Ai Hua Fu, Zhu Jun Zhang, Li Li Chen, Xiao Ming Zhang, Pan Xue. High performance liquid chromatography with immobilized Ru(bpy)32+-KMnO4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes[J]. Chinese Chemical Letters, 2011, 22(10): 1245-1248. doi: 10.1016/j.cclet.2011.05.023
Citation: Ai Hua Fu, Zhu Jun Zhang, Li Li Chen, Xiao Ming Zhang, Pan Xue. High performance liquid chromatography with immobilized Ru(bpy)32+-KMnO4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes[J]. Chinese Chemical Letters, 2011, 22(10): 1245-1248. doi: 10.1016/j.cclet.2011.05.023
High performance liquid chromatography with immobilized Ru(bpy)32+-KMnO4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes
摘要:
A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH2PO4 (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO4) and tris (2, 2'-bipyridyl)ruthenium (III) (Ru (bpy)33+), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.
English
High performance liquid chromatography with immobilized Ru(bpy)32+-KMnO4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes
Abstract:
A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH2PO4 (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO4) and tris (2, 2'-bipyridyl)ruthenium (III) (Ru (bpy)33+), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.
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