引用本文:
Xue Ping Ji, Xian Rui Li, Na Wang, Rui Xing Ni, Xiao Hong Liu, Hua Ai Xiong. Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor[J]. Chinese Chemical Letters,
2010, 21(10): 1239-1242.
doi:
10.1016/j.cclet.2010.05.022
Citation: Xue Ping Ji, Xian Rui Li, Na Wang, Rui Xing Ni, Xiao Hong Liu, Hua Ai Xiong. Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor[J]. Chinese Chemical Letters, 2010, 21(10): 1239-1242. doi: 10.1016/j.cclet.2010.05.022
Citation: Xue Ping Ji, Xian Rui Li, Na Wang, Rui Xing Ni, Xiao Hong Liu, Hua Ai Xiong. Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor[J]. Chinese Chemical Letters, 2010, 21(10): 1239-1242. doi: 10.1016/j.cclet.2010.05.022
Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor
摘要:
A mixed self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic acid amide (T-NH2) were used to immobilize tyrosinase for fabricating biosensor. The results showed that the mixed SAMs prepared from solution at the ratio of 1:4provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate. The biosensor exhibited a fast response and high sensitivity for sensing substrate.
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关键词:
- Biosensor
- / Tyrosinase
- / Catechol
- / Self-assembled monolayers
- / Gold electrode
English
Attachment of tyrosinase on mixed self-assembled monolayers for the construction of electrochemical biosensor
Abstract:
A mixed self-assembled monolayers (SAMs) of thioctic acid (T-COOH) and thioctic acid amide (T-NH2) were used to immobilize tyrosinase for fabricating biosensor. The results showed that the mixed SAMs prepared from solution at the ratio of 1:4provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate. The biosensor exhibited a fast response and high sensitivity for sensing substrate.
-
Key words:
- Biosensor
- / Tyrosinase
- / Catechol
- / Self-assembled monolayers
- / Gold electrode
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