引用本文:
Yin Yan Zhang, Zi Cheng Li, Jin Kun Zhu, Zhi Yong Yang, Qing Jiang Wang, Pin Gang He, Yu Zhi Fang. Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection[J]. Chinese Chemical Letters,
2010, 21(10): 1231-1234.
doi:
10.1016/j.cclet.2010.04.040
Citation: Yin Yan Zhang, Zi Cheng Li, Jin Kun Zhu, Zhi Yong Yang, Qing Jiang Wang, Pin Gang He, Yu Zhi Fang. Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection[J]. Chinese Chemical Letters, 2010, 21(10): 1231-1234. doi: 10.1016/j.cclet.2010.04.040
Citation: Yin Yan Zhang, Zi Cheng Li, Jin Kun Zhu, Zhi Yong Yang, Qing Jiang Wang, Pin Gang He, Yu Zhi Fang. Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection[J]. Chinese Chemical Letters, 2010, 21(10): 1231-1234. doi: 10.1016/j.cclet.2010.04.040
Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection
摘要:
A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer (1.5×10-2mol/L borate-3×10-2 mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90 V (vs. SCE) and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10-7 to 2.1×10-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.
English
Simultaneous determination of flavonoids and anthraquinones in chrysanthemum by capillary electrophoresis with amperometry detection
Abstract:
A high-performance capillary electrophoresis with amperometry detection method (CE-AD) has been developed for the analysis of flavonoids and anthraquinones (emodin, kaempferol, apigenin, luteolin and rhein) in chrysanthemum. Under optimum conditions, these five analytes were base-line separated within 17 min using a borate-phosphate running buffer (1.5×10-2mol/L borate-3×10-2 mol/L phosphate running buffer, pH 9.0) at a working potential of +0.90 V (vs. SCE) and a separation voltage of 19 kV. The linear relationship between concentration and current response was obtained with detection limits (S/N=3) ranging from 1.0×10-7 to 2.1×10-7 g/mL for all analytes. This proposed method was successfully used in the analysis of four kinds of chrysanthemum with relatively simple extraction procedures, the assay results were satisfactory.
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Key words:
- Capillary electrophoresis
- / Amperometry detection
- / Chrysanthemu
- / Flavonoids
- / Anthraquinones
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