引用本文:
Jiang Hua Zhong, Peng Qin, Wei Sun, Kui Jiao. Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels[J]. Chinese Chemical Letters,
2008, 19(10): 1244-1247.
doi:
10.1016/j.cclet.2008.06.036
Citation: Jiang Hua Zhong, Peng Qin, Wei Sun, Kui Jiao. Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels[J]. Chinese Chemical Letters, 2008, 19(10): 1244-1247. doi: 10.1016/j.cclet.2008.06.036
Citation: Jiang Hua Zhong, Peng Qin, Wei Sun, Kui Jiao. Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels[J]. Chinese Chemical Letters, 2008, 19(10): 1244-1247. doi: 10.1016/j.cclet.2008.06.036
Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels
摘要:
An electrochemical method for the simultaneous detection of two different DNA sequenees from PAT and FMV 35S gene sequence using CdS and PbS quantum dots (QDs)as labels was described.The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate.The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method(DPASV).The DPASV signals of the oxidation of Cd2+ and Pb2+ ions present in the solution were difierent and reflected the identity of corresponding ssDNA targets sequences.
English
Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels
Abstract:
An electrochemical method for the simultaneous detection of two different DNA sequenees from PAT and FMV 35S gene sequence using CdS and PbS quantum dots (QDs)as labels was described.The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate.The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method(DPASV).The DPASV signals of the oxidation of Cd2+ and Pb2+ ions present in the solution were difierent and reflected the identity of corresponding ssDNA targets sequences.
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