A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

Citation: Shui-bin Feng, De-hao Fu, Lei Nie, Peng Zou, Jin-ping Suo. A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method[J]. Chinese Journal of Polymer Science, 2015, 33(7): 955-963. doi: 10.1007/s10118-015-1660-9 shu

A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

1.
State Key Laboratory of Material Processing and Die and Mould Technology, College of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
2.
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
基金项目:
This work was financially supported by the Independent Innovation Foundation of Huazhong University of Science and Technology (No. 2013ZHYX008) and the National Natural Science Foundation of China (No. 81370980).

摘要: PLGA, mPEG diblock copolymer was synthesized by bulk ring-opening polymerization method. The double emulsion solvent evaporation method was used to prepare bovine serum albumin (BSA)-loaded microspheres. Optical microscopy was used to observe the whole microsphere fabrication process. It is confirmed that the proportion of inner aqueous phase is one of the most critical factors that determines the morphology of microspheres. Double emulsion droplets which have appropriate amount of inner aqueous phase can form closed and dense microspheres, while, too much inner aqueous phase will cause a collapse of the double emulsion droplets, resulting in a loss of drug. The proportion of inner aqueous phase was varied to prepare microspheres of different morphology. The results show that with increasing the amount of inner aqueous phase, a higher percent of broken microspheres and lower encapsulation efficiency appeared, and also, a more severe initial burst release and faster release rate.

关键词:

English

A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

1.
State Key Laboratory of Material Processing and Die and Mould Technology, College of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
2.
Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
Received Date: 23 October 2014
Revised Date: 10 March 2015
Available Online: 05 July 2015
Fund Project:
This work was financially supported by the Independent Innovation Foundation of Huazhong University of Science and Technology (No. 2013ZHYX008) and the National Natural Science Foundation of China (No. 81370980).

Abstract: PLGA, mPEG diblock copolymer was synthesized by bulk ring-opening polymerization method. The double emulsion solvent evaporation method was used to prepare bovine serum albumin (BSA)-loaded microspheres. Optical microscopy was used to observe the whole microsphere fabrication process. It is confirmed that the proportion of inner aqueous phase is one of the most critical factors that determines the morphology of microspheres. Double emulsion droplets which have appropriate amount of inner aqueous phase can form closed and dense microspheres, while, too much inner aqueous phase will cause a collapse of the double emulsion droplets, resulting in a loss of drug. The proportion of inner aqueous phase was varied to prepare microspheres of different morphology. The results show that with increasing the amount of inner aqueous phase, a higher percent of broken microspheres and lower encapsulation efficiency appeared, and also, a more severe initial burst release and faster release rate.

Key words:

1. [1]
  Wang, M., Feng, Q., Niu, X., Tan, R. and She, Z., Polym. Degrad. Stab., 2010, 95(1): 6
2. [2]
  Ho, M.L., Fu, Y.C., Wang, G.J., Chen, H.T., Chang, J.K., Tsai, T.H. and Wang, C.K., J. Control. Release, 2008, 128(2): 142
3. [3]
  Kakizawa, Y., Nishio, R., Hirano, T., Koshi, Y., Nukiwa, M., Koiwa, M., Michizoe, J. and Ida, N., J. Control. Release, 2010, 142(1): 8
4. [4]
  Bae, S.E., Son, J.S., Park, K. and Han, D.K., J. Control. Release, 2009, 133(1): 37
5. [5]
  Yeh, M. and Chiang, C., J. Microencapsulation, 2004, 21(1): 91
6. [6]
  Gasparini, G., Holdich, R.G. and Kosvintsev, S.R., Colloids Surf., B, 2010, 75(2): 557
7. [7]
  Anderson, J.M. and Shive, M.S., Adv. Drug Delivery Rev., 1997, 28(1): 5
8. [8]
  De la Ossa, D.H.P., Ligresti, A., Gil-Alegre, M.E., Aberturas, M.R., Molpeceres, J., Di Marzo, V. and Suarez, A.I.T., J. Control. Release, 2012, 161(3): 927
9. [9]
  Yoo, N.Y., Youn, Y.S., Oh, N.M., Oh, K.T., Lee, D.K., Cha, K.H., Oh, Y.T. and Lee, E.S., Colloids Surf., B, 2011, 88(1): 419
10. [10]
  Lecaroz, C., Gamazo, C., Renedo, M.J. and Blanco-Prieto, M.J., J. Microencapsulation, 2006, 23(7): 782
11. [11]
  Karal-Yilmaz, O., Serhatli, M., Baysal, K. and Baysal, B.M., J. Microencapsulation, 2011, 28(1): 46
12. [12]
  Fude, C., Dongmei, C., Anjin, T., Mingshi, Y., Kai, S., Min, Z. and Ying, G., J. Control. Release, 2005, 107(2): 310
13. [13]
  Zhou, S.B., Liao, X.Y., Li, X.H., Deng, X.M. and Li, H., J. Control. Release, 2003, 86(2-3): 195
14. [14]
  Li, J., Jiang, G. and Ding, F., J. Appl. Polym. Sci., 2008, 108(4): 2458
15. [15]
  Lee, S.Y., Hyun, H., Youn, J.Y., Kim, B.S., Song, I.B., Kim, M.S., Lee, B., Khang, G. and Lee, H.B., Colloids Surf., A, 2008, 313-314: 126
16. [16]
  Thomasin, C., Ho, N.T., Merkle, H.P. and Gander, B., J. Pharm. Sci., 1998, 87(3): 259
17. [17]
  Yang, Y.Y., Chung, T.S. and Ng, N.P., Biomaterials, 2001, 22(3): 231
18. [18]
  Tao Y., Zhang, H.L., Hu, Y.M., Wan, S. and Su, Z.Q., Int. J. Mol. Sci., 2013, 14(2): 4174
19. [19]
  Yang, X.Z., Dou, S., Sun, T.M., Mao, C.Q., Wang, H.X. and Wang, J., J. Control. Release, 2011, 156(2): 203
20. [20]
  Beletsi, A., Leontiadis, L., Klepetsanis, P., Ithakissios, D.S. and Avgoustakis, K., Int. J. Pharm., 1999, 182(2): 187
21. [21]
  Yang, Y.Y., Chung, T.S., Bai, X.L. and Chan, W.K., Chem. Eng. Sci., 2000, 55(12): 2223
22. [22]
  Petros, R.A. and DeSimone, J.M., Nat. Rev. Drug Discov., 2010, 9(8): 615
23. [23]
  Jiang, H.H., Kim, T.H., Lee, S., Chen, X., Youn, Y.S. and Lee, K.C., Biomaterials, 2011, 32(33): 8529
24. [24]
  Bajgai, M.P., Aryal, S., Parajuli, D.C., Khil, M.S., Lee, D.R. and Kim, H.Y., J. Appl. Polym. Sci., 2009, 111(3): 1540
25. [25]
  Meng, F.T., Ma, G.H., Liu, Y.D., Qiu, W. and Su, Z.G., Colloids Surf., B, 2004, 33(3-4): 177
26. [26]
  Zou, P., Suo, J.P., Nie, L. and Feng, S.B., J. Mater. Chem., 2012, 22(13): 6316
27. [27]
  Zou, P., Suo, J. P., Nie, L. and Feng, S.B., Polymer, 2012, 53(6): 1245
28. [28]
  Rosca, I.D., Watari, F. and Uo, M., J. Control. Release, 2004, 99(2): 271

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沈阳化工大学材料科学与工程学院沈阳 110142

A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

English

A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

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A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

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A Detailed View of PLGA-mPEG Microsphere Formation by Double Emulsion Solvent Evaporation Method

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