Effect of Tetrabutylammonium Bromide on Enzymatic Polymerization of Phenol Catalyzed by Horseradish Peroxidase

Hua Duan Ke Zheng Yuan-chen Cui Yu-dong Li Lei Zhang

Citation:  Hua Duan, Ke Zheng, Yuan-chen Cui, Yu-dong Li, Lei Zhang. Effect of Tetrabutylammonium Bromide on Enzymatic Polymerization of Phenol Catalyzed by Horseradish Peroxidase[J]. Chinese Journal of Polymer Science, 2014, 32(7): 906-913. doi: 10.1007/s10118-014-1473-2 shu

Effect of Tetrabutylammonium Bromide on Enzymatic Polymerization of Phenol Catalyzed by Horseradish Peroxidase

    通讯作者: Yuan-chen Cui,
  • 基金项目:

    This work was financially supported by the Natural Science Foundation of Henan Province (No. 132300410147).

摘要: In this article tetrabutylammonium bromide (TBAB) was first added in buffer to compose a convenient and environmentally friendly system, and enzymatic polymerization of phenol catalyzed by horseradish peroxidase (HRP) could proceed efficiently in this system. When TBAB was added, the most conversion of phenol could reach 99.1%. The phenol polymer was considered to consist of a mixture of phenylene (Ph) and oxyphenylene (Ox) units by IR analysis, and the ratio of phenylene to oxyphenylene units (Ph/Ox) was measured by titration. Moreover, the effects of the dosage of horseradish peroxidase (HRP) and pH value on the conversion of phenol were investigated. The reaction performed very effectively in this novel system when the addition of HRP was only 0.2 mg. In all cases, the weight-average molecular weight calculated by GPC-SLS was in a range from 12000 Da to 30000 Da. The phenol polymer prepared in the present research possessed good thermal stability shown by TG analysis.

English


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  • 发布日期:  2014-07-05
  • 收稿日期:  2013-10-24
  • 修回日期:  2014-02-25
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