引用本文:
Jing XU, Lan Hua ZHAO, Xin Guo WU, Hong Mei WANG, Ru Xiu CAI. New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry[J]. Chinese Chemical Letters,
2006, 17(7): 949-952.
Citation: Jing XU, Lan Hua ZHAO, Xin Guo WU, Hong Mei WANG, Ru Xiu CAI. New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry[J]. Chinese Chemical Letters, 2006, 17(7): 949-952.
Citation: Jing XU, Lan Hua ZHAO, Xin Guo WU, Hong Mei WANG, Ru Xiu CAI. New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry[J]. Chinese Chemical Letters, 2006, 17(7): 949-952.
New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry
摘要:
A highly sensitive stopped-flow spectrophotometry kinetic method was proposed forquantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide (NADH). Phenoxyl radicals generated from as low as 1×10-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10-9mol/L.
English
New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry
Abstract:
A highly sensitive stopped-flow spectrophotometry kinetic method was proposed forquantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide (NADH). Phenoxyl radicals generated from as low as 1×10-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10-9mol/L.
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Key words:
- Phenoxyl radicals
- / moleculary probe
- / stopped-flow spectrophotometry
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