引用本文:
Yan Jing SU, Xiong Hui WEI. Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA[J]. Chinese Chemical Letters,
2006, 17(5): 691-694.
Citation: Yan Jing SU, Xiong Hui WEI. Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA[J]. Chinese Chemical Letters, 2006, 17(5): 691-694.
Citation: Yan Jing SU, Xiong Hui WEI. Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA[J]. Chinese Chemical Letters, 2006, 17(5): 691-694.
Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA
摘要:
The binding of chelerythrine (CHE) iminium to calf-thymus (CT) DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA.These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×105 and a binding site size of 3, 4 base pairs at 25℃.The intercalative interaction is exothermic with a Van't Hoff enthalpy of -33.7 kJ/mol. In addition, △G=-32.9 kJ/mol, and △S=-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.
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关键词:
- Chelerythrine
- / CT-DNA
- / intercalation
- / thermodynamic
English
Interaction of Chelerythrine Choloride in Acid Buffer with Calf-thymus DNA
Abstract:
The binding of chelerythrine (CHE) iminium to calf-thymus (CT) DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA.These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×105 and a binding site size of 3, 4 base pairs at 25℃.The intercalative interaction is exothermic with a Van't Hoff enthalpy of -33.7 kJ/mol. In addition, △G=-32.9 kJ/mol, and △S=-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.
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Key words:
- Chelerythrine
- / CT-DNA
- / intercalation
- / thermodynamic
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