引用本文:
Gu Ping TANG, Zhi Yu WANG. Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro[J]. Chinese Chemical Letters,
2006, 17(1): 113-116.
Citation: Gu Ping TANG, Zhi Yu WANG. Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro[J]. Chinese Chemical Letters, 2006, 17(1): 113-116.
Citation: Gu Ping TANG, Zhi Yu WANG. Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro[J]. Chinese Chemical Letters, 2006, 17(1): 113-116.
Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro
摘要:
The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa.Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5(mol/mol). The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.
English
Polyethylenimine (PEI)-g-comb-poly(ethylene glycol)-transferrin(Ⅰ):Tumor-targeted Vector for Gene Delivery In-vitro
Abstract:
The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa.Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5(mol/mol). The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.
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Key words:
- PEI-g-Comb-PEG
- / transferrin
- / gene therapy
- / transfection efficiency
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