引用本文:
Ying Hua QIU, Al Jin HUANG, Yi Liang SUN. Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis[J]. Chinese Chemical Letters,
1999, 10(3): 227-230.
Citation: Ying Hua QIU, Al Jin HUANG, Yi Liang SUN. Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis[J]. Chinese Chemical Letters, 1999, 10(3): 227-230.
Citation: Ying Hua QIU, Al Jin HUANG, Yi Liang SUN. Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis[J]. Chinese Chemical Letters, 1999, 10(3): 227-230.
Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis
摘要:
Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis.Sufficiently high column efficiencies (>2×105 plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time.The relationship between the adsorption suppression effect of the modifier and its structure was discussed.
English
Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis
Abstract:
Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis.Sufficiently high column efficiencies (>2×105 plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time.The relationship between the adsorption suppression effect of the modifier and its structure was discussed.
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Key words:
- Capillary zone electrophoresis
- / basic proteins
- / dynamic modifier
- / Triethylamne
- / diethylenetriamine
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