引用本文:
Tong LIU, Xin Du GENG. The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography[J]. Chinese Chemical Letters,
1999, 10(3): 219-222.
Citation: Tong LIU, Xin Du GENG. The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography[J]. Chinese Chemical Letters, 1999, 10(3): 219-222.
Citation: Tong LIU, Xin Du GENG. The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography[J]. Chinese Chemical Letters, 1999, 10(3): 219-222.
The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography
摘要:
The separation efficiency of biopolymers with a short column in liquid chromatography has been investigated in this paper.It was found that the column length has slight effect on the resolution of biopolymers under gradient elution.The reasons have been explained by stoichiometric displacement model for retention of solute.The column 1 .0 cm long was also used in the separation and purification of recombinant human granulocyte colony-simulating factor (rhG-CSF).It only took 40 min and the purity by one step was found to be almost 100%.
English
The Separation Efficiency of Biopolymers with Short Column in Liquid Chromatography
Abstract:
The separation efficiency of biopolymers with a short column in liquid chromatography has been investigated in this paper.It was found that the column length has slight effect on the resolution of biopolymers under gradient elution.The reasons have been explained by stoichiometric displacement model for retention of solute.The column 1 .0 cm long was also used in the separation and purification of recombinant human granulocyte colony-simulating factor (rhG-CSF).It only took 40 min and the purity by one step was found to be almost 100%.
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Key words:
- Short column
- / biopolymer
- / stoichiometric displacement model for retention
- / Z value
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