引用本文:
Wen Ke FENG, Xin Du GENG. SYNTHESIS OF INTERFERON-αA MONOCLONAL ANTIBODY PACKING MATERIAL IN HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PURIFICATION OF RECOMBINANT HUMAN INTERFERON-αA[J]. Chinese Chemical Letters,
1991, 2(5): 383-386.
Citation: Wen Ke FENG, Xin Du GENG. SYNTHESIS OF INTERFERON-αA MONOCLONAL ANTIBODY PACKING MATERIAL IN HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PURIFICATION OF RECOMBINANT HUMAN INTERFERON-αA[J]. Chinese Chemical Letters, 1991, 2(5): 383-386.
Citation: Wen Ke FENG, Xin Du GENG. SYNTHESIS OF INTERFERON-αA MONOCLONAL ANTIBODY PACKING MATERIAL IN HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PURIFICATION OF RECOMBINANT HUMAN INTERFERON-αA[J]. Chinese Chemical Letters, 1991, 2(5): 383-386.
SYNTHESIS OF INTERFERON-αA MONOCLONAL ANTIBODY PACKING MATERIAL IN HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PURIFICATION OF RECOMBINANT HUMAN INTERFERON-αA
摘要:
A new way for the synthesis of human interferon-αA monoclonal antibody (IFN-αA-McAb) bound to silica gel packing material in high-performance affinity chromatography (HPAFC) has been developed. The high coupling efficiency and specific activity of IFN-αA-McAb can be obtained by activated diol-silica gel with activating agent. After purification using this packing material in HPAFC, the specific activity of recombinant human interferon-αA (rIFN-αA) rose up to 1.03×107IU/mg protein and the purification efficiency is appoximately 100 times.
English
SYNTHESIS OF INTERFERON-αA MONOCLONAL ANTIBODY PACKING MATERIAL IN HIGH-PERFORMANCE AFFINITY CHROMATOGRAPHY AND PURIFICATION OF RECOMBINANT HUMAN INTERFERON-αA
Abstract:
A new way for the synthesis of human interferon-αA monoclonal antibody (IFN-αA-McAb) bound to silica gel packing material in high-performance affinity chromatography (HPAFC) has been developed. The high coupling efficiency and specific activity of IFN-αA-McAb can be obtained by activated diol-silica gel with activating agent. After purification using this packing material in HPAFC, the specific activity of recombinant human interferon-αA (rIFN-αA) rose up to 1.03×107IU/mg protein and the purification efficiency is appoximately 100 times.
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